Vespa G N, Cunha F Q, Silva J S
Department of Immunology, Faculty of Medicine of Ribeirão Preto, São Paulo, Brazil.
Infect Immun. 1994 Nov;62(11):5177-82. doi: 10.1128/iai.62.11.5177-5182.1994.
This study was carried out to determine the role of reactive nitrogen intermediates in Trypanosoma cruzi infection. In vitro, splenocytes obtained during the acute phase of infection produced elevated amounts of nitric oxide (NO) that were correlated with the resistance or susceptibility of the animals. In vivo, the levels of NO2- plus NO3- in plasma during the later phase of infection were higher in C57BL/6 mice than in BALBL/c mice. The treatment of infected C57BL/6 mice with inhibitors of NO synthase increased parasitemia and mortality. Finally, we found that the NO donor drug S-nitroso-acetyl-penicillamine is able to kill trypomastigotes in vitro in the absence of any other cells, suggesting a direct NO-mediated killing of T. cruzi.
本研究旨在确定活性氮中间体在克氏锥虫感染中的作用。在体外，感染急性期获得的脾细胞产生的一氧化氮（NO）量增加，这与动物的抗性或易感性相关。在体内，感染后期C57BL/6小鼠血浆中NO2-加NO3-的水平高于BALBL/c小鼠。用NO合酶抑制剂治疗感染的C57BL/6小鼠会增加寄生虫血症和死亡率。最后，我们发现NO供体药物S-亚硝基乙酰青霉胺在没有任何其他细胞的情况下能够在体外杀死锥鞭毛体，这表明NO直接介导对克氏锥虫的杀伤作用。
