PAb1614，一种与SV40、JC、BK和多瘤病毒的肿瘤抗原发生反应的单克隆抗体，以及PAb1601 - 1636组中的其他JC病毒肿瘤抗原交叉反应抗体。

PAb1614, a monoclonal antibody reactive with the tumor antigens of SV40, JC, BK, and polyoma virus, and other JC virus tumor antigen cross-reactive antibodies of the PAb1601-1636 panel.

作者信息

Hess R, Oberbeck J, Grussenmeyer T, Schoeffel-Keller A, Haessler C, Braun D G, Brandner G

机构信息

Abteilung Virologie, Universität Freiburg, BRD.

出版信息

Intervirology. 1994;37(1):47-52. doi: 10.1159/000150356.

DOI:10.1159/000150356

PMID:7523331

Abstract

PAb1614, an SV40-specific monoclonal antibody of the panel PAb1601-1636 reacts with large and small tumor antigens of SV40, BK and JC virus, and with polyoma virus large and middle tumor antigens, but not with the large tumor antigen of the lymphotropic papova virus. Using immunofluorescence and immunoblot competition assays and ELISA with synthetic peptides, it is shown that the epitope is represented by the SV40 tumor antigen undecapeptide, K39-E49. This peptide comprises the tumor antigen consensus sequence, H42-G47, of the polyoma viruses. However, the epitope of PAb1614 probably does not exactly coincide with this hexapeptide. This explains why some cross-reactions are less strong, or absent, as in the case of the lymphotropic papova virus. Further antibodies of the PAb1601-1636 panel that cross-react with the JC virus large tumor antigen are PAb1602, 1604, 1606, 1618, 1621, 1622, 1623, 1624, 1626, 1629, and 1633.

摘要

PAb1614是PAb1601 - 1636组中的一种SV40特异性单克隆抗体，可与SV40、BK和JC病毒的大小肿瘤抗原以及多瘤病毒的大、中肿瘤抗原发生反应，但不与嗜淋巴细胞性多瘤病毒的大肿瘤抗原发生反应。通过免疫荧光、免疫印迹竞争试验以及使用合成肽的ELISA表明，该表位由SV40肿瘤抗原十一肽K39 - E49代表。此肽包含多瘤病毒的肿瘤抗原共有序列H42 - G47。然而，PAb1614的表位可能与该六肽并不完全一致。这就解释了为什么一些交叉反应较弱或不存在，比如嗜淋巴细胞性多瘤病毒的情况。PAb1601 - 1636组中其他与JC病毒大肿瘤抗原发生交叉反应的抗体有PAb1602、1604、1606、1618、1621、1622、1623、1624、1626、1629和1633。