Chen W D, Jia P C, Chen S S, Liang C C, Wang B S, Gao S, Wang X J, Wang M, Qu S K
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Hemoglobin. 1994 May;18(3):193-9. doi: 10.3109/03630269409043619.
The G gamma:A gamma ratios of Hb F were determined in 331 newborn babies of the Man ethnic group in Liaoning Province, P.R. China, using polyacrylamide gel electrophoresis; in 27 cases they were also determined by reversed phase high performance liquid chromatography. The results showed that a high G gamma value (> 80%) was present in four cases (1.21%) and low G gamma values (30-48%) in six cases (1.81%); not a single case with the A gamma T [A gamma 75(E19)Ile->Thr] chain was found. Gene mapping analysis demonstrated that two babies with high G gamma values had the -G gamma-G gamma-/-G gamma-A gamma- (0.60%), and two others the -G gamma-AG gamma-A gamma-/-G gamma-A gamma- (0.60%) arrangements. Two of the four babies with low G gamma values were identified as -A gamma-A gamma-/-G gamma-A gamma- (0.60%) and the other two as -GA gamma-/-G gamma-A gamma- (0.60%). The genotype -A gamma-A gamma- has not been reported before in Chinese.
采用聚丙烯酰胺凝胶电泳法测定了中国辽宁省满族331例新生儿的Hb F的Gγ∶Aγ比值；其中27例同时采用反相高效液相色谱法进行测定。结果显示，4例（1.21%）出现高Gγ值（>80%），6例（1.81%）出现低Gγ值（30%～48%）；未发现1例携带AγT [Aγ75（E19）Ile→Thr]链。基因图谱分析表明，2例高Gγ值婴儿的基因型为-Gγ-Gγ-/-Gγ-Aγ-（0.60%），另外2例为-Gγ-AGγ-Aγ-/-Gγ-Aγ-（0.60%）。4例低Gγ值婴儿中，2例被鉴定为-Aγ-Aγ-/-Gγ-Aγ-（0.60%），另外2例为-GAγ-/-Gγ-Aγ-（0.60%）。-Aγ-Aγ-基因型在中国此前未见报道。
