Sindic C J, Monteyne P, Laterre E C
Laboratory of Neurochemistry, Catholic University of Louvain, Cliniques Universitaires Saint-Luc, Brussels, Belgium.
J Neuroimmunol. 1994 Oct;54(1-2):75-80. doi: 10.1016/0165-5728(94)90233-x.
A highly sensitive antigen-mediated capillary blot technique was developed for the detection of virus-specific oligoclonal IgG in paired CSF and serum samples from patients with various neurological diseases. In multiple sclerosis, intrathecal synthesis of oligoclonal antibodies was present against measles (70%), rubella (60%), varicella zoster (40%) and mumps (30%); in most cases (75%), such synthesis involved two or more viruses. In contrast, antibodies against a non-neurotropic virus (cytomegalovirus) were rarely produced in CSF from MS patients (5%). However, this 'polyspecific' reaction was not restricted to MS samples but was also observed in neurolupus and in the late phase of infectious diseases of the central nervous system. These anti-viral antibodies could be produced without de novo replication of the corresponding viral genome and are likely mere bystanders of an ongoing immune response.
我们开发了一种高灵敏度的抗原介导毛细管印迹技术，用于检测患有各种神经疾病患者的配对脑脊液和血清样本中病毒特异性寡克隆IgG。在多发性硬化症中，存在针对麻疹（70%）、风疹（60%）、水痘带状疱疹（40%）和腮腺炎（30%）的鞘内寡克隆抗体合成；在大多数情况下（75%），这种合成涉及两种或更多种病毒。相比之下，MS患者脑脊液中很少产生针对非嗜神经病毒（巨细胞病毒）的抗体（5%）。然而，这种“多特异性”反应并不局限于MS样本，在神经狼疮和中枢神经系统传染病的晚期也有观察到。这些抗病毒抗体的产生可能无需相应病毒基因组的重新复制，可能仅仅是正在进行的免疫反应的旁观者。
