用抗105-kD分子抗体诱导小鼠B细胞增殖并保护其免于凋亡：X连锁免疫缺陷B细胞的无反应性
Murine B cell proliferation and protection from apoptosis with an antibody against a 105-kD molecule: unresponsiveness of X-linked immunodeficient B cells.
作者信息
Miyake K, Yamashita Y, Hitoshi Y, Takatsu K, Kimoto M
机构信息
Department of Immunology, Saga Medical School, Japan.
出版信息
J Exp Med. 1994 Oct 1;180(4):1217-24. doi: 10.1084/jem.180.4.1217.
Abstract
We established a novel monoclonal antibody, RP/14, that can protect B cells from apoptosis induced by irradiation or dexamethasone. A molecule recognized by RP/14 (the RP antigen) was expressed on B cells with B220bright, IgMdull, and IgDbright. Immunoprecipitation experiments revealed that RP/14 recognized a monomeric protein with an approximate molecular mass of 105 kD. Stimulation of B cells with RP/14 for 48 h induced B cell proliferation and blastogenesis. In contrast to B cells of wild-type mice, X-linked immunodeficient (XID) B cells did not proliferate upon stimulation with RP/14, although the RP antigen was expressed to the same extent as that of wild-type B cells. These results suggest that the RP antigen-mediated signaling pathway is important for rescuing B cells from apoptosis and is deficient in XID B cells.
摘要
我们制备了一种新型单克隆抗体RP/14，它能够保护B细胞免受辐射或地塞米松诱导的凋亡。RP/14识别的分子（RP抗原）在B220bright、IgMdull和IgDbright的B细胞上表达。免疫沉淀实验表明，RP/14识别一种分子量约为105 kD的单体蛋白。用RP/14刺激B细胞48小时可诱导B细胞增殖和母细胞化。与野生型小鼠的B细胞不同，X连锁免疫缺陷（XID）B细胞在用RP/14刺激后不增殖，尽管RP抗原的表达程度与野生型B细胞相同。这些结果表明，RP抗原介导的信号通路对于挽救B细胞免于凋亡很重要，且在XID B细胞中存在缺陷。
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