Skinner S M, Killian G J, Miller L A, Dunbar B S
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
J Reprod Fertil. 1994 Jul;101(2):295-303. doi: 10.1530/jrf.0.1010295.
The effectiveness of zona pellucida antigens in immunizing white-tailed deer to reduce fertility was evaluated by analysing the constituent deer zona pellucida proteins and their immunogenicity. Does were immunized with porcine zona pellucida antigens. The antibodies were characterized using immunohistochemical and immunoblot analysis, in which zona pellucida proteins were separated by one-dimensional and two-dimensional PAGE. Deer anti-porcine zona pellucida antibodies were found to recognize all the major proteins of the porcine zona pellucida. These antibodies also recognized several proteins of deer zona pellucida, indicating that it is possible to break immune tolerance in the deer using such a protocol. The antibodies were also found to recognize peptides of 55 and 75 kDa that were produced by expressing cDNA clones containing antigens of major glycoproteins of rabbit zona pellucida. Furthermore, antibodies against rabbit zonae pellucidae recognized antigens in zonae of paraffin-embedded deer ovaries. Taken together, these experiments demonstrate the crossreactive nature of a number of zona pellucida epitopes found in deer and in several other species. They also illustrate the immunogenicity possible in such an immunization protocol, and provide valuable probes for the investigation of follicular development in this and other species.
通过分析白尾鹿透明带蛋白的组成及其免疫原性，评估了透明带抗原对白尾鹿进行免疫以降低生育力的有效性。用猪透明带抗原对母鹿进行免疫。使用免疫组织化学和免疫印迹分析对抗体进行表征，其中透明带蛋白通过一维和二维聚丙烯酰胺凝胶电泳进行分离。发现鹿抗猪透明带抗体能够识别猪透明带的所有主要蛋白质。这些抗体还识别了鹿透明带的几种蛋白质，这表明使用这样的方案有可能打破鹿的免疫耐受。还发现这些抗体能够识别通过表达含有兔透明带主要糖蛋白抗原的cDNA克隆所产生的55 kDa和75 kDa的肽。此外，抗兔透明带抗体能够识别石蜡包埋的鹿卵巢透明带中的抗原。综上所述，这些实验证明了在鹿和其他几个物种中发现的许多透明带表位具有交叉反应性。它们还说明了这种免疫方案中可能存在的免疫原性，并为研究该物种和其他物种的卵泡发育提供了有价值的探针。
