Gonsebatt M E, Vega L, Montero R, Garcia-Vargas G, Del Razo L M, Albores A, Cebrian M E, Ostrosky-Wegman P
Instituto de Investigaciones Biomédicas, UNAM, DF, Mexico.
Mutat Res. 1994 Oct-Dec;313(2-3):293-9. doi: 10.1016/0165-1161(94)90059-0.
A human monitoring study was carried out to explore the effect on lymphocyte proliferation of chronic exposure to arsenic (As) via drinking water. Blood and urine samples were taken from volunteers from a town where levels of As in the drinking water averaged 412 micrograms/l, and from a matched group of individuals, with similar socioeconomic status, that drank water with As average levels of 37.2 micrograms/l. Exposure was assessed by questionnaires and by determining the levels of As in urine and water samples. The evaluation of the peripheral blood lymphocyte proliferation was done at different culture times using labelling (LI), mitotic (MI) and replication indexes (RI) as endpoints. No significant differences were seen for either LI or MI, except for MI in 72 h cultures and in LI in males and females with skin lesions vs. those without lesions. Significant differences in RI were seen for exposed females but not for males. Correlations between LI and MI showed that progression from the initial S-to M-phase is altered in exposed individuals. Arsenic exposure as well as lead and mercury affect cellular immune response, making the endpoints of cell proliferation variables of interest in population monitoring study design, since they might provide information in health impairment due to exposure, which is important in risk assessment.
开展了一项人体监测研究，以探究通过饮用水长期接触砷（As）对淋巴细胞增殖的影响。采集了来自一个城镇志愿者的血液和尿液样本，该城镇饮用水中的砷含量平均为412微克/升，还采集了来自一组社会经济地位相似的对照个体的样本，他们饮用的水中砷平均含量为37.2微克/升。通过问卷调查以及测定尿液和水样中的砷含量来评估暴露情况。使用标记指数（LI）、有丝分裂指数（MI）和复制指数（RI）作为终点指标，在不同培养时间对外周血淋巴细胞增殖进行评估。LI和MI均未观察到显著差异，但在72小时培养的MI以及有皮肤病变与无皮肤病变的男性和女性的LI中除外。暴露女性的RI存在显著差异，而男性则无。LI和MI之间的相关性表明，暴露个体从初始S期到M期的进程发生了改变。砷暴露以及铅和汞会影响细胞免疫反应，使得细胞增殖变量的终点指标在人群监测研究设计中成为重要关注点，因为它们可能提供因暴露导致健康损害的信息，这在风险评估中很重要。
