Zhu J, Link H, Mix E, Olsson T, Huang W X
Department of Neurology, Karolinska Institute, Huddinge Hospital, Stockholm, Sweden.
Acta Neurol Scand. 1994 Jul;90(1):19-25. doi: 10.1111/j.1600-0404.1994.tb02674.x.
Experimental allergic neuritis (EAN) is a T cell-mediated animal model of Guillain-Barré syndrome characterized by inflammation and demyelination of peripheral nerves. EAN can be induced by immunization of rats with bovine peripheral nerve myelin (BPM) or the myelin proteins P2 or P0, but the extent of T cell responses over the course of EAN is incompletely defined. We studied the T cell responses to these proteins and the glycolipid GM1 by enumerating T helper type 1 (Th1)-like cells secreting interferon-gamma (IFN-gamma) after short-term culture of mononuclear cells (MNC) in presence of antigen. Already 7 days post immunization (p.i.) with BPM and before onset of clinical EAN, lymph nodes contained elevated levels of P2 responsive T cells. At the height of EAN on day 14 p.i. and during recovery, T cell levels responding to BPM, P0 and GM1 were also elevated. The same temporal profiles and specificities were registered for antigen reactive spleen MNC. The results implicate that Th1-like cells with multiple specificities including the glycolipid GM1 occur at increased levels in lymphoid organs in EAN rats, and that IFN-gamma may be an important effector molecule in the induction of nerve damage.
实验性变应性神经炎（EAN）是一种由T细胞介导的格林-巴利综合征动物模型，其特征为外周神经的炎症和脱髓鞘。用牛外周神经髓磷脂（BPM）或髓磷脂蛋白P2或P0免疫大鼠可诱发EAN，但EAN病程中T细胞反应的程度尚未完全明确。我们通过在抗原存在的情况下对单核细胞（MNC）进行短期培养后，枚举分泌干扰素-γ（IFN-γ）的1型辅助性T细胞（Th1样细胞），研究了T细胞对这些蛋白和糖脂GM1的反应。在用BPM免疫后7天且临床EAN发作之前，淋巴结中对P2有反应的T细胞水平就已升高。在免疫后第14天EAN高峰期及恢复期间，对BPM、P0和GM1有反应的T细胞水平也升高。抗原反应性脾MNC也呈现相同的时间模式和特异性。结果表明，具有包括糖脂GM1在内的多种特异性的Th1样细胞在EAN大鼠的淋巴器官中水平升高，并且IFN-γ可能是诱导神经损伤的重要效应分子。
