Virta E, Salo A, Uusitalo H
Department of Anatomy, University of Helsinki, Finland.
Int J Dev Neurosci. 1994 Jun;12(3):175-83. doi: 10.1016/0736-5748(94)90039-6.
The effect of isoprenaline, carbachol, substance P (SP) and neurokinin A (NKA) on peroxidase and total protein secretion was studied in the developing postnatal submandibular glands of the rat using in vitro methods. Submandibular glands of 1, 5, 12 and 30 day-old rats were stimulated by 10(-5) M isoprenaline and carbachol, and 10(-6) M SP and NKA. The stimulatory effects of these compounds were compared to the basic release of peroxidase and total amount of protein from submandibular gland fragments in incubation solution with no added transmitter substances. Indirect immunohistochemical methods were used to study these developing glands from SP- and NKA-immunoreactive (IR) nerve fibers. The distributions of SP-IR and NKA-IR nerve fibers closely resembled each other, being most abundantly spread around the developing acini and ducts. The number of these fibers was high on the 1st, 5th and 12th days, but was decreased on the 30th day. On peroxidase release, isoprenaline was the most effective, causing a maximal response of 47 times the basic release on the first postnatal day, after which it gradually decreased. The effects of carbachol, SP and NKA on peroxidase release were clearly weaker and, unlike isoprenaline, their strongest response was on the 5th postnatal day (carbachol, 4.3; SP 5.2; NKA, 4.5). The total protein secretion effect patterns of the studied substances resembled each other more, showing their strongest response on the 5th day (isoprenaline, 5.0; carbachol, 4.5; SP, 4.2; NKA, 3.4) and decreasing thereafter.(ABSTRACT TRUNCATED AT 250 WORDS)
采用体外方法研究了异丙肾上腺素、卡巴胆碱、P物质（SP）和神经激肽A（NKA）对新生大鼠下颌下腺过氧化物酶和总蛋白分泌的影响。用10⁻⁵M异丙肾上腺素、卡巴胆碱以及10⁻⁶M SP和NKA刺激1日龄、5日龄、12日龄和30日龄大鼠的下颌下腺。将这些化合物的刺激作用与未添加递质物质的孵育溶液中下颌下腺碎片过氧化物酶的基础释放量和总蛋白量进行比较。采用间接免疫组织化学方法研究这些发育中的腺体中SP和NKA免疫反应性（IR）神经纤维的情况。SP-IR和NKA-IR神经纤维的分布彼此非常相似，在发育中的腺泡和导管周围分布最为丰富。这些纤维的数量在第1天、第5天和第12天较高，但在第30天减少。在过氧化物酶释放方面，异丙肾上腺素最为有效，在出生后第一天引起的最大反应是基础释放量的47倍，此后逐渐下降。卡巴胆碱、SP和NKA对过氧化物酶释放的作用明显较弱，与异丙肾上腺素不同，它们最强的反应出现在出生后第5天（卡巴胆碱为4.3；SP为5.2；NKA为4.5）。所研究物质的总蛋白分泌效应模式彼此更相似，在第5天表现出最强反应（异丙肾上腺素为5.0；卡巴胆碱为4.5；SP为4.2；NKA为3.4），此后逐渐下降。（摘要截短于250字）
