Virta E, Kangas S, Tolonen R, Schultz T, Salo A, Uusitalo H
Department of Anatomy, University of Helsinki, Finland.
Acta Physiol Scand. 1991 Jun;142(2):157-63. doi: 10.1111/j.1748-1716.1991.tb09143.x.
An indirect immunofluorescence technique was used to study the distribution of neurokinin A immunoreactive (NKA-IR) nerve fibres in submandibular and parotid glands of the rat. The functional role of neurokinin A on protein and peroxidase secretion in these glands was evaluated by using in vitro methods. In the parotid gland neurokinin A immunoreactive fibres were mainly distributed around the secretory acini, but some were also in evidence around the stromal blood vessels and ducts. The number of the neurokinin A immunoreactive nerve fibres was lower in the submandibular gland than in the parotid gland. They were mainly distributed around the secretory acini and stromal blood vessels and ducts. In vitro, neurokinin A significantly stimulated the release of total amount of released proteins and peroxidase from parotid gland fragments, while in the submandibular gland only the release of peroxidase was increased. By using SDS polyacrylamide gel electrophoresis (SDS-PAGE) specific changes were found in the release of proteins after neurokinin A stimulation. The results of the present study demonstrate that neurokinin A immunoreactive nerve fibres are present in the rat parotid and submandibular glands. Their localization around the secretory elements of the glands and the effect of neurokinin A in vitro experiments indicates that neurokinin A might have a significant role in the regulation of salivary secretion.
采用间接免疫荧光技术研究大鼠颌下腺和腮腺中神经激肽A免疫反应性（NKA-IR）神经纤维的分布。通过体外实验方法评估神经激肽A对这些腺体中蛋白质和过氧化物酶分泌的功能作用。在腮腺中，神经激肽A免疫反应性纤维主要分布在分泌性腺泡周围，但在基质血管和导管周围也有一些。颌下腺中神经激肽A免疫反应性神经纤维的数量比腮腺中的少。它们主要分布在分泌性腺泡、基质血管和导管周围。在体外，神经激肽A显著刺激腮腺组织块中释放的蛋白质总量和过氧化物酶的释放，而在颌下腺中仅过氧化物酶的释放增加。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）发现，神经激肽A刺激后蛋白质释放有特定变化。本研究结果表明，大鼠腮腺和颌下腺中存在神经激肽A免疫反应性神经纤维。它们在腺体分泌元件周围的定位以及神经激肽A在体外实验中的作用表明，神经激肽A可能在唾液分泌调节中起重要作用。
