Törnwall J, Uusitalo H, Konttinen Y T
Department of Anatomy, University of Helsinki, Finland.
Cell Tissue Res. 1994 Aug;277(2):309-13. doi: 10.1007/BF00327778.
The distribution and origin of substance P (SP) and neurokinin A (NKA) were studied in rat in the anterior buccal glands, which are minor mucous salivary glands. Indirect immunofluorescence staining showed moderate SP and NKA innervation of salivary acini and interlobular ducts, whereas blood vessels were more sparsely innervated, and there were few nerve fibers in the stroma and around the intralobular ducts. About 10%-20% of the trigeminal ganglion cells showed equally strong immunoreactivity to both SP and NKA. Unilateral denervation of the branches of the trigeminal nerve caused complete disappearance of the stromal fibers and greatly reduced the number of all other SP-immunoreactive and NKA-immunoreactive nerve fibers. In the superior cervical ganglia, SP and NKA immunoreactivity was restricted to small intensely fluorescent cells; SP and NKA immunoreactivity was absent from principal ganglionic cells, and thus sympathectomy had no any effect on the number or distribution of fibers immunoreactive for SP and NKA in the anterior buccal glands. The fibers remaining after sensory denervation could have been of parasympathetic origin, indicating a dual origin of nerves immunoreactive for SP and NKA in these glands. The present data demonstrate that the major part of the glandular SP and NKA innervation in the minor salivary glands derives from the trigeminal ganglia. The distribution of the peripheral nerve fibers indicates that they may play a role in the delivery of potent neuropeptides involved in the vascular, secretory, and motor (myoepithelial cells) functions of salivary glands.
研究了大鼠颊前腺（一种小黏液唾液腺）中P物质（SP）和神经激肽A（NKA）的分布及起源。间接免疫荧光染色显示，唾液腺泡和小叶间导管有中等程度的SP和NKA神经支配，而血管的神经支配较为稀疏，在间质和小叶内导管周围神经纤维较少。约10%-20%的三叉神经节细胞对SP和NKA显示出同样强的免疫反应性。三叉神经分支的单侧去神经支配导致间质纤维完全消失，并使所有其他SP免疫反应性和NKA免疫反应性神经纤维数量大幅减少。在颈上神经节中，SP和NKA免疫反应性局限于小而强荧光细胞；主神经节细胞中不存在SP和NKA免疫反应性，因此交感神经切除术对颊前腺中SP和NKA免疫反应性纤维的数量或分布没有任何影响。感觉去神经支配后残留的纤维可能来自副交感神经，表明这些腺体中SP和NKA免疫反应性神经有双重起源。目前的数据表明，小唾液腺中腺性SP和NKA神经支配的主要部分来自三叉神经节。外周神经纤维的分布表明，它们可能在传递参与唾液腺血管、分泌和运动（肌上皮细胞）功能的强效神经肽中发挥作用。
