Kitajima I, Kawahara K, Nakajima T, Soejima Y, Matsuyama T, Maruyama I
Department of Laboratory Medicine, Faculty of Medicine, Kagoshima University, Japan.
Biochem Biophys Res Commun. 1994 Oct 14;204(1):244-51. doi: 10.1006/bbrc.1994.2451.
To investigate how the number of mast cells is controlled, we studied a murine mastocytoma cell line. Based on electron microscopic observation of nuclear condensation and electrophoretic evidence with DNA fragmentation, these mastocytoma wells were shown to undergo apoptosis. This apoptosis was dependent on the concentrations of serum and L-arginine and was enhanced by TNF-alpha. We confirmed that apoptosis was mediated by nitric oxide (NO) synthase; inducible NO synthase (iNOS) mRNA was strongly expressed in apoptotic cells, while an inhibitor of NOS, NG-monomethyl-L-arginine, and dexamethasone prevented apoptosis in addition to inhibiting iNOS mRNA expression. Our results suggest that iNOS expression is very important in regulating the proliferation of mast cells under pathological conditions.
为了研究肥大细胞数量是如何被控制的，我们研究了一种小鼠肥大细胞瘤细胞系。基于对核浓缩的电子显微镜观察以及DNA片段化的电泳证据，这些肥大细胞瘤孔被证明会发生凋亡。这种凋亡依赖于血清和L-精氨酸的浓度，并被肿瘤坏死因子-α增强。我们证实凋亡是由一氧化氮（NO）合酶介导的；诱导型NO合酶（iNOS）mRNA在凋亡细胞中强烈表达，而一氧化氮合酶抑制剂NG-单甲基-L-精氨酸和地塞米松除了抑制iNOS mRNA表达外，还能阻止凋亡。我们的结果表明，iNOS表达在病理条件下调节肥大细胞增殖中非常重要。
