Senar S, Recio M N, Pérez-Albarsanz M A
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
Cell Signal. 1994 May;6(4):433-8. doi: 10.1016/0898-6568(94)90090-6.
The ability of lindane to change the metabolism of inositol phospholipids was investigated using rat renal proximal tubular cell cultures labelled with [3H]inositol. Lindane addition to the culture medium caused labelling of inositol trisphosphate, inositol bisphosphate and inositol tetrakisphosphate to decrease and that of the inositol monophosphate pool to increase. A depletion of radioactivity in phosphatidylinositols was also observed after lindane addition. Most strikingly, the addition of lindane considerably increased the levels of glycerophosphoinositol in a dose-dependent manner. The effect of lindane follows a different pattern from that of bradykinin, and it is suggested to act by stimulating phospholipase A activity(ies).
使用用[³H]肌醇标记的大鼠肾近端小管细胞培养物，研究了林丹改变肌醇磷脂代谢的能力。向培养基中添加林丹导致三磷酸肌醇、二磷酸肌醇和四磷酸肌醇的标记减少，而单磷酸肌醇池的标记增加。添加林丹后还观察到磷脂酰肌醇中的放射性耗竭。最显著的是，添加林丹以剂量依赖的方式显著增加了甘油磷酸肌醇的水平。林丹的作用模式与缓激肽不同，提示其通过刺激磷脂酶A活性起作用。
