Puente-Fraga J C, López-Aparicio P, Senar S, Recio M N, Pérez-Albarsanz M A
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Alcala de Henares-Madrid, Spain.
Biosci Rep. 1995 Aug;15(4):191-9. doi: 10.1007/BF01540453.
Gamma- and delta-isomers of hexachlorocyclohexane caused marked decreases in the levels of radioactive phospholipids, and increases in the levels of [3H]arachidonate incorporated into free fatty acids in rat renal tubular cells. The increased radioactivity of free fatty acids arises from the decrease of [3H]arachidonate incorporated into phosphatidylinositol, but not into phosphatidylcholine, phosphatidylserine or phosphatidylethanolamine. This fact suggests that phosphatidylinositol can be broken down to the fatty acid from the sn-2 position and lysophospholipid by a phospholipase activity increased by hexachlorocyclohexanes. The observed specific toxicant action could be achieved in two ways: (a) operating upon a specific phospholipase A2 that acts on phosphatidylinositol, but not on other phospholipids as substrates and/or (b) involving substrate-phospholipase A2 interactions. Interestingly, the observed effect of the delta-isomer was more pronounced than that of the gamma-one.
六氯环己烷的γ-异构体和δ-异构体可使大鼠肾小管细胞中放射性磷脂水平显著降低，并使掺入游离脂肪酸中的[3H]花生四烯酸水平升高。游离脂肪酸放射性的增加源于掺入磷脂酰肌醇而非磷脂酰胆碱、磷脂酰丝氨酸或磷脂酰乙醇胺的[3H]花生四烯酸的减少。这一事实表明，磷脂酰肌醇可通过六氯环己烷增强的磷脂酶活性从sn-2位分解为脂肪酸和溶血磷脂。观察到的特定毒物作用可通过两种方式实现：（a）作用于一种特定的磷脂酶A2，该酶作用于磷脂酰肌醇，但不作用于其他磷脂作为底物，和/或（b）涉及底物-磷脂酶A2相互作用。有趣的是，观察到的δ-异构体的作用比γ-异构体更为明显。
