Minc-Golomb D, Schwartz J P
Clinical Neuroscience Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Neuroreport. 1994 Jul 21;5(12):1466-8. doi: 10.1097/00001756-199407000-00014.
Nitric oxide (NO), a short-lived, highly diffusible free radical, is a messenger molecule produced through the conversion of arginine to citrulline by NO synthase (NOS). In the CNS, NO functions as an important neuromodulator. We now report that cerebellar granule cells in culture express the constitutive form of NOS (cNOS). The expression is demonstrated both at the level of RNA, by RNA-specific reverse transcription-polymerase chain reaction (RT-PCR), and of protein, by immunostaining and by enzymatic activity. Cerebellar granule cells can thus serve as a tool to study the regulation of expression and activity of cNOS in a defined environment.
一氧化氮（NO）是一种半衰期短、扩散性强的自由基，是由一氧化氮合酶（NOS）将精氨酸转化为瓜氨酸而产生的信使分子。在中枢神经系统中，NO作为一种重要的神经调节剂发挥作用。我们现在报告，培养的小脑颗粒细胞表达组成型NOS（cNOS）。通过RNA特异性逆转录聚合酶链反应（RT-PCR）在RNA水平以及通过免疫染色和酶活性在蛋白质水平均证实了这种表达。因此，小脑颗粒细胞可作为一种工具，用于研究在特定环境中cNOS表达和活性的调节。
