Sato I, Kim Y, Himi T, Murota S
Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.
Neurosci Lett. 1995 Jan 23;184(2):145-8. doi: 10.1016/0304-3940(94)11191-k.
Cultured cerebellar granule neurons were assayed for nitric oxide synthase (NOS) activity by measuring the conversion of L-arginine to L-citrulline. Granule neurons expressed constitutive NOS activity which was calcium-dependent. Treatment of neuron cultures for 24 h with the combined stimulation of IFN-gamma plus IL-1 beta, TNF-alpha and LPS induced NOS activity by 87-fold which was calcium-independent. We conclude that cultured cerebellar granule neurons can express both the constitutive and inducible forms of NOS activity.
