Mouradov A, Mouradova E, Scott K J
Department of Biochemistry, University of Queensland, Brisbane, Australia.
Plant Mol Biol. 1994 Oct;26(1):503-7. doi: 10.1007/BF00039561.
A genomic (prb1) and two cDNA clones (PRb1-2 and PRb1-3) corresponding to two new barley basic PR-1 proteins (prb1-2 and prb1-3) were isolated from Hordeum vulgare. Genomic analysis of DNA suggests that the barley genome contains at least 6 members corresponding to the gene family encoding PR-1 proteins. Expression of these genes was induced in primary leaf tissues of the H. vulgare cv. Psaknon 4* (F14) Man. carrying Mlp resistance gene (cv. Mlp) and the near-isogenic susceptible cultivar (cv. mlp) after inoculation with Erysiphe graminis f. sp. hordei.
从大麦中分离出了与两种新的大麦碱性病程相关蛋白1(prb1-2和prb1-3)对应的一个基因组(prb1)和两个cDNA克隆(PRb1-2和PRb1-3)。DNA的基因组分析表明,大麦基因组至少包含6个与编码病程相关蛋白1的基因家族对应的成员。在用禾本科布氏白粉菌大麦专化型接种后,这些基因在携带Mlp抗性基因(品种Mlp)的大麦品种Psaknon 4*(F14)Man.和近等基因感病品种(品种mlp)的初生叶组织中被诱导表达。