Matsushita A, Nagai K, Ishikawa T, Tatsumi E, Ohno Y, Takahashi T
Department of Immuno-Hematology, Kobe City General Hospital, Japan.
Int J Hematol. 1994 Aug;60(2):157-61.
Although the expression of myeloid-associated antigen CD13 has been reported in aggressive B-cell chronic lymphocytic leukemia, its expression in other mature B-cell neoplasms appears to be rare. We report a 74-year-old female with B-cell prolymphocytic leukemia (B-PLL) expressing CD13 antigen. On admission, splenomegaly was noted. Hematological examination revealed a platelet count of 90 x 10(9)/l and a white cell count of 68 x 10(9)/l with 73% PLL cells. The hemoglobin concentration was 10.6 g/dl. A bone marrow aspirate showed a normocellular marrow with 64% PLL cells. Surface marker analysis of the PLL cells was positive for CD11b, CD13, CD19, CD20, CD24, HLA-DR, FMC7, mu and lambda. Simultaneous expression of CD13 and CD19 antigen was confirmed by dual color flow cytometry. Southern blot analysis of DNA from circulating mononuclear cells gave a rearranged band for the immunoglobulin gene (JH) but not for TCR-beta. Cytogenetic analysis of marrow cells showed an abnormal karyotype involving numbers 1, 7, 10, 12, 14, 15 chromosomes.
尽管有报道称髓系相关抗原CD13在侵袭性B细胞慢性淋巴细胞白血病中表达，但其在其他成熟B细胞肿瘤中的表达似乎很少见。我们报告了一名74岁表达CD13抗原的B细胞幼淋巴细胞白血病（B-PLL）女性患者。入院时发现脾肿大。血液学检查显示血小板计数为90×10⁹/L，白细胞计数为68×10⁹/L，其中幼淋巴细胞占73%。血红蛋白浓度为10.6 g/dl。骨髓穿刺显示骨髓细胞正常，幼淋巴细胞占64%。幼淋巴细胞的表面标志物分析显示CD11b、CD13、CD19、CD20、CD24、HLA-DR、FMC7、μ和λ呈阳性。双色流式细胞术证实了CD13和CD19抗原的同时表达。对循环单核细胞DNA的Southern印迹分析显示免疫球蛋白基因（JH）有重排条带，但TCR-β无重排条带。骨髓细胞的细胞遗传学分析显示核型异常，涉及1、7、10、12、14、15号染色体。
