促红细胞生成素在肝细胞癌中的基因表达。

Gene expression of erythropoietin in hepatocellular carcinoma.

作者信息

Muta H, Funakoshi A, Baba T, Uike N, Wakasugi H, Kozuru M, Jimi A

机构信息

Department of Gastroenterology, National Kyushu Cancer Center, Fukuoka.

出版信息

Intern Med. 1994 Jul;33(7):427-31. doi: 10.2169/internalmedicine.33.427.

DOI:10.2169/internalmedicine.33.427

PMID:7524823

Abstract

A 68-year-old man with hepatocellular carcinoma complicated by erythrocytosis showed an increased plasma level of immunoreactive erythropoietin (EPO). Northern blot analysis and RT-PCR (reverse transcriptase and polymerase chain reaction) of EPO mRNA extracted from a surgical specimen indicated high expression of EPO mRNA in the tumor tissue. Histological and immunocytochemical examination showed that the tumor was a hepatocellular carcinoma with predominant immunostaining for EPO. The erythrocytosis improved and the high serum EPO level decreased after resection of the tumor. This is the first demonstration of EPO mRNA expression in hepatocellular carcinoma tissue by RT-PCR.

摘要

一名68岁肝细胞癌合并红细胞增多症的男性患者，其血浆中免疫反应性促红细胞生成素（EPO）水平升高。对手术标本中提取的EPO mRNA进行Northern印迹分析和RT-PCR（逆转录酶和聚合酶链反应），结果显示肿瘤组织中EPO mRNA高表达。组织学和免疫细胞化学检查表明，该肿瘤为肝细胞癌，对EPO有主要免疫染色。肿瘤切除后，红细胞增多症改善，血清EPO高水平下降。这是首次通过RT-PCR证明EPO mRNA在肝细胞癌组织中的表达。