Omote Y, Ikeda K, Yamazaki K, Kubo Y
First Department of Surgery, Asahikawa Medical College, Japan.
Surg Today. 1994;24(7):616-20. doi: 10.1007/BF01833726.
We evaluated three solutions used for preserving lungs, namely, University of Wisconsin (UW), Euro-Collins (E-C), and low potassium dextran (LPD), by measuring the high energy phosphates in the preserved lung tissue. The left lungs of Sprague-Dawley rats were excised and flushed with 5 ml of one of the solutions at 10 degrees C through the pulmonary artery, after which they were deflated and immersed in the solution at 10 degrees C for 24 h. The tissue adenosine triphosphate (ATP) concentration in mumol/g tissue wet weight after 24 h of storage was 2.55 +/- 0.48 (n = 7) in the UW lungs, 1.98 +/- 0.25 (n = 6) in the E-C lungs, and 1.53 +/- 0.32 (n = 4) in the LPD lungs, being significantly higher in the UW lungs than in either the E-C or LPD lungs (P < 0.05). The histopathological findings of the E-C lungs were more deteriorated, with marked interstitial edema, septal hypertrophy, and perivascular hyaline degeneration, than either the UW or LPD lungs. Thus, the findings of this study indicate the superiority of UW solution for lung preservation.
我们通过测量保存的肺组织中的高能磷酸盐，评估了三种用于保存肺的溶液，即威斯康星大学（UW）溶液、欧洲柯林斯（E-C）溶液和低钾右旋糖酐（LPD）溶液。将Sprague-Dawley大鼠的左肺切除，并在10℃下通过肺动脉用5毫升上述溶液之一进行冲洗，然后放气并在10℃的溶液中浸泡24小时。储存24小时后，UW组肺组织中每克湿重组织的三磷酸腺苷（ATP）浓度为2.55±0.48（n = 7），E-C组肺为1.98±0.25（n = 6），LPD组肺为1.53±0.32（n = 4），UW组肺中的ATP浓度显著高于E-C组和LPD组（P < 0.05）。E-C组肺的组织病理学表现比UW组和LPD组更差，有明显的间质水肿、间隔增厚和血管周围透明变性。因此，本研究结果表明UW溶液在肺保存方面具有优越性。
