Hausen B, Beuke M, Schroeder F, Poets C F, Hewitt C, DelRossi A J, Schäfers H J
Division of Thoracic and Cardiovascular Surgery, Surgical Center, Hannover Medical School, Germany.
Eur J Cardiothorac Surg. 1997 Nov;12(5):771-9; discussion 779-80. doi: 10.1016/s1010-7940(97)00205-4.
The impact of storage solution composition on graft performance was evaluated following perfusion with either Euro-Collins (EC), low potassium Euro-Collins (rEC), low potassium dextran (LPD) or University of Wisconsin solution (UW) after brief (2 h) and extended ischemia (16 h) in an acute double lung transplantation model in the rat.
Following flush perfusion and ischemia the lungs were implanted in recipient rats allowing serial assessment of graft pulmonary vascular resistance (PVR) and alveolar arterial oxygen difference (AaDO2) during 120 min of reperfusion. Graft dynamic lung compliance (DLC) was determined by separate ventilation. Final evaluation included weight gain and histology.
After extended ischemia LPD provided superior graft function in respect to DLC (repeated measures ANOVA; LPD versus rEC P < 0.05; versus EC P < 0.03; versus UW P < 0.05) and AaDO2 (LPD versus rEC P < 0.04; versus EC P < 0.006). The PVR was significantly lower in LPD versus UW (P < 0.05). At the end of reperfusion the weight increase amounted to 229 +/- 49% in rEC, 207 +/- 22% in EC, 115 +/- 22% in UW and 87 + 17% in LPD (LPD versus rEC P < 0.01, LPD versus EC P < 0.001). The type of preservation solution used had little impact on graft function after 2 h ischemia.
Low potassium dextran provides superior graft function after extended ischemia. After short ischemia the type of preservation solution used in this study had little impact on global lung function.
在大鼠急性双肺移植模型中，对肺脏进行短暂（2小时）和延长缺血（16小时）后，用欧洲柯林斯液（EC）、低钾欧洲柯林斯液（rEC）、低钾右旋糖酐液（LPD）或威斯康星大学液（UW）灌注，评估保存液成分对移植肺功能的影响。
冲洗灌注和缺血后，将肺脏植入受体大鼠体内，在再灌注120分钟期间连续评估移植肺的肺血管阻力（PVR）和肺泡动脉氧分压差（AaDO2）。通过单独通气测定移植肺的动态肺顺应性（DLC）。最终评估包括重量增加情况和组织学检查。
延长缺血后，LPD在DLC方面提供了更好的移植肺功能（重复测量方差分析；LPD与rEC相比P<0.05；与EC相比P<0.03；与UW相比P<0.05）以及AaDO2方面（LPD与rEC相比P<0.04；与EC相比P<0.006）。LPD组的PVR显著低于UW组（P<0.05）。再灌注结束时，rEC组重量增加达229±49%，EC组为207±22%，UW组为115±22%，LPD组为87±17%（LPD与rEC相比P<0.01，LPD与EC相比P<0.001）。缺血2小时后，所用保存液的类型对移植肺功能影响不大。
低钾右旋糖酐在延长缺血后能提供更好的移植肺功能。短暂缺血后，本研究中所用保存液的类型对整体肺功能影响不大。
