Leigh B R, Webb S, Hancock S L, Knox S J
Department of Radiation Oncology, Stanford University Medical Center, California.
Stem Cells. 1994 Jul;12(4):430-5. doi: 10.1002/stem.5530120411.
The effect of recombinant human stem cell factor (SCF) on the response of human fetal bone marrow progenitor cells to irradiation was studied using immunodeficient mice with human fetal bone grafts (SCID/Hu mice). SCID/Hu mice were treated with three intraperitoneal injections of 500 micrograms/kg SCF at 20 h before, two h before, and four h after 100 cGy total body irradiation. Fourteen days following irradiation, the fetal bone grafts were harvested and studied. Most of the isolated bone marrow cells were human, as determined by flow cytometry. Colony forming assays were performed on the bone marrow to determine the survival of erythroid (BFU-E) and myeloid (CFU-GM) precursor cells. A statistically significant increase in BFU-E and CFU-GM survival after irradiation was observed for bone marrow maintained in the SCF treated mice when compared to bone marrow from mice not treated with SCF. The enhancement in colony forming unit survival after irradiation ranged from 4.3-fold for BFU-E (p = 0.05) to 13.1-fold for CFU-GM (p = 0.002). These findings suggest that SCF may be of potential clinical value for the prevention of radiation-induced myelosuppression.
利用植入人胎儿骨的免疫缺陷小鼠（SCID/Hu小鼠），研究了重组人干细胞因子（SCF）对人胎儿骨髓祖细胞辐射反应的影响。在全身照射100 cGy前20小时、前2小时及照射后4小时，给SCID/Hu小鼠腹腔注射三次500微克/千克的SCF。照射后14天，采集胎儿骨移植物并进行研究。通过流式细胞术测定，分离出的大多数骨髓细胞为人类细胞。对骨髓进行集落形成试验，以确定红系（BFU-E）和髓系（CFU-GM）前体细胞的存活情况。与未用SCF处理的小鼠的骨髓相比，观察到在经SCF处理的小鼠中维持的骨髓在照射后BFU-E和CFU-GM存活有统计学意义的增加。照射后集落形成单位存活的增强倍数，BFU-E为4.3倍（p = 0.05），CFU-GM为13.1倍（p = 0.002）。这些发现表明，SCF对于预防辐射诱导的骨髓抑制可能具有潜在的临床价值。
