Leigh B R, Hancock S L, Knox S J
Department of Radiation Oncology, Stanford University Hospital, California 94305.
Cancer Res. 1993 Sep 1;53(17):3857-9.
This study evaluates the effect of recombinant human stem cell factor (SCF) on the in vitro response of human bone marrow progenitor cells to irradiation. Light density nonadherent mononuclear cells were isolated from human bone marrow and resuspended in either semisolid culture or liquid culture with or without 100 ng/ml SCF. After 24 h in culture, cells were irradiated and assessed for survival of erythroid burst-forming unit, granulocyte colony-forming unit(s), or granulocyte-macrophage colony-forming unit precursors in the presence of erythropoietin, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor, respectively. Incubation with SCF prior to irradiation (0-300 cGy) resulted in an increase in both absolute colony number and surviving fraction for erythroid burst-forming units, granulocyte colony-forming units, and granulocyte-macrophage colony-forming units as compared to cultures that did not contain SCF. The mean surviving fraction enhancement ratio after 100 cGy ranged from 1.2 to 3.7. An increased fraction of CD34+ progenitors in S-phase after exposure to SCF may explain in part the apparent radioprotective effect of SCF on human bone marrow progenitor cells.
本研究评估重组人干细胞因子（SCF）对人骨髓祖细胞体外辐射反应的影响。从人骨髓中分离低密度非贴壁单核细胞，并将其重悬于含或不含100 ng/ml SCF的半固体培养或液体培养体系中。培养24小时后，对细胞进行辐照，并分别在促红细胞生成素、粒细胞集落刺激因子或粒细胞-巨噬细胞集落刺激因子存在的情况下，评估红系爆式集落形成单位、粒细胞集落形成单位或粒细胞-巨噬细胞集落形成单位前体的存活情况。与不含SCF的培养物相比，在辐照（0 - 300 cGy）前用SCF孵育可导致红系爆式集落形成单位、粒细胞集落形成单位和粒细胞-巨噬细胞集落形成单位的绝对集落数和存活分数均增加。100 cGy辐照后的平均存活分数增强比在1.2至3.7之间。暴露于SCF后S期CD34 +祖细胞比例增加可能部分解释了SCF对人骨髓祖细胞的明显辐射防护作用。
