Harper S B, Hurst W J, Lang C M
Department of Comparative Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
J Chromatogr B Biomed Appl. 1994 Jul 15;657(2):339-44. doi: 10.1016/s0378-4347(94)80007-3.
A capillary electrophoretic technique was developed to monitor patterns of hemoglobin production in young calves subjected to multiple phlebotomies. The method is similar to one previously described for the determination of human hemoglobin variants in whole blood using isoelectric focusing. After a single collection of one-half the circulating blood volume, there were obvious alterations in hemoglobin chain variants. HbA levels diminished as blood loss increased with minimum values corresponding to maximum blood loss. HbF levels did not appear to be affected. Also visible during the regenerative process were atypical overlapping peaks preceding the normal hemoglobin peaks. At the conclusion of the 18-day study, most of the electropherograms had returned to initial states. These changes were found to be a sensitive indicator of accelerated erythropoiesis in contrast to the standard technique of total hemoglobin determination by colorimetric means.
开发了一种毛细管电泳技术，用于监测多次采血的幼犊血红蛋白产生模式。该方法类似于先前描述的使用等电聚焦法测定全血中人类血红蛋白变体的方法。单次采集一半循环血量后，血红蛋白链变体出现明显变化。随着失血量增加，HbA水平降低，最低值对应最大失血量。HbF水平似乎未受影响。在再生过程中还可见到正常血红蛋白峰之前的非典型重叠峰。在为期18天的研究结束时，大多数电泳图谱已恢复到初始状态。与通过比色法测定总血红蛋白的标准技术相比，发现这些变化是加速红细胞生成的敏感指标。
