急性心肌梗死患者中P-选择素的可溶性形式。
Soluble form of P-selectin in patients with acute myocardial infarction.
作者信息
Ikeda H, Nakayama H, Oda T, Kuwano K, Muraishi A, Sugi K, Koga Y, Toshima H
机构信息
Third Department of Internal Medicine, Kurume University School of Medicine, Japan.
出版信息
Coron Artery Dis. 1994 Jun;5(6):515-8.
BACKGROUND
P-selectin, an integral membrane glycoprotein of platelets and endothelial cells, is rapidly redistributed to the cell surface after cellular activation. The soluble form of P-selectin has been shown to be present in people with normal circulation. The purpose of the present study was therefore to examine the soluble form of P-selectin in patients with acute myocardial infarction (AMI).
METHODS
Whole blood was obtained from nine patients with AMI and from 10 volunteers who made up the control group. Plasma concentrations of the soluble form of P-selectin were examined with a monoclonal antibody-based enzyme immunoassay.
RESULTS
Plasma P-selectin levels in control volunteers were 178 +/- 44 ng/ml. In patients with AMI, plasma P-selectin levels on days 1, 2 and 3 were 743 +/- 374, 627 +/- 267, and 588 +/- 223 ng/ml, respectively (P < 0.001)--significantly higher than the levels in the control volunteers.
CONCLUSION
Plasma concentrations of the soluble form of P-selectin were markedly higher in patients with AMI, suggesting the activation of platelets, or endothelial cells, or both. Thus, quantitative measurements of plasma P-selectin concentrations may help to assess the pathophysiology of inflammatory reactions in patients with AMI.
背景
P-选择素是血小板和内皮细胞的一种整合膜糖蛋白，细胞活化后会迅速重新分布到细胞表面。已证明正常循环的人体内存在可溶性P-选择素。因此，本研究的目的是检测急性心肌梗死（AMI）患者的可溶性P-选择素。
方法
从9例AMI患者和10名作为对照组的志愿者中采集全血。采用基于单克隆抗体的酶免疫分析法检测可溶性P-选择素的血浆浓度。
结果
对照组志愿者的血浆P-选择素水平为178±44 ng/ml。AMI患者第1、2和3天的血浆P-选择素水平分别为743±374、627±267和588±223 ng/ml（P<0.001），显著高于对照组志愿者的水平。
结论
AMI患者血浆中可溶性P-选择素的浓度明显更高，提示血小板或内皮细胞或两者均被激活。因此，定量检测血浆P-选择素浓度可能有助于评估AMI患者炎症反应的病理生理学。
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