Frankfurt M, McKittrick C R, Luine V N
Department of Neuroendocrinology, Rockefeller University, New York, NY 10021.
Brain Res. 1994 Jul 4;650(1):127-32. doi: 10.1016/0006-8993(94)90214-3.
The effects of short-term fluoxetine administration on monoamine levels and turnover were assessed in discrete brain nuclei. Adult male rats received fluoxetine HCl (10 mg/kg) or saline injections intraperitoneally for 4 days and monoamine levels determined by high performance liquid chromatography. The major metabolite of 5-HT, 5-hydroxyindoleacetic acid (5-HIAA), was decreased by fluoxetine treatment in the ventromedial hypothalamic nucleus (VMN), the lateral hypothalamic area and the CA1 region of the hippocampus. Fluoxetine treatment significantly increased serotonin (5-HT) levels in the VMN but did not change 5-HT levels in any other area examined. Norepinephrine (NE) levels were higher in fluoxetine-treated rats in the dorsomedial hypothalamic nucleus, dorsal raphe nucleus and parietal motor cortex (MCTX). 5-HT and NE turnover were also determined by the pargyline method. Fluoxetine treatment decreased 5-HT turnover in the VMN and increased 5-HT turnover in the median raphe. NE turnover was decreased in the preoptic area, the MCTX and parietal sensory cortex by fluoxetine administration. These results demonstrate that brain areas with similar 5-HT innervation respond differently to fluoxetine administration and fluoxetine, which selectively alters 5-HT uptake, also affects NE levels and turnover in several brain nuclei.
在离散的脑核中评估了短期给予氟西汀对单胺水平和周转率的影响。成年雄性大鼠腹腔注射盐酸氟西汀（10mg/kg）或生理盐水，持续4天，并通过高效液相色谱法测定单胺水平。在腹内侧下丘脑核（VMN）、下丘脑外侧区和海马体CA1区，氟西汀治疗使5-羟色胺（5-HT）的主要代谢产物5-羟吲哚乙酸（5-HIAA）减少。氟西汀治疗显著提高了VMN中的5-HT水平，但在其他任何检测区域均未改变5-HT水平。在氟西汀治疗的大鼠中，背内侧下丘脑核、中缝背核和顶叶运动皮层（MCTX）中的去甲肾上腺素（NE）水平较高。还通过帕吉林法测定了5-HT和NE的周转率。氟西汀治疗降低了VMN中的5-HT周转率，并增加了中缝正中核中的5-HT周转率。氟西汀给药使视前区、MCTX和顶叶感觉皮层中的NE周转率降低。这些结果表明，具有相似5-HT神经支配的脑区对氟西汀给药的反应不同，并且选择性改变5-HT摄取的氟西汀也会影响几个脑核中的NE水平和周转率。
