Jónsdóttir I, Luthman M, Ekre H P, Skoog B, Brostedt P, Roos P, Werner S, Perlmann P
Department of Immunology, University of Stockholm, Sweden.
Horm Res. 1994;41(5-6):197-204. doi: 10.1159/000183892.
Human growth hormone (hGH) was analyzed by six monoclonal antibodies (Mabs) and a polyclonal antiserum (Pas) before and after molecular sieve chromatography of sera from healthy subjects. Their hGH levels were between < 0.2 and 0.4 ng/ml as determined with Pas. The six Mabs reacted with five distinct epitopes and bound to a hGH fragment corresponding to the amino acid sequence 15-125. Two of the Mabs showed reduced binding to 20-kD hGH. The binding of Mabs to dimeric forms of hGH varied. Human GH levels in unfractionated sera as determined with Mabs were < 3.1-390 ng/ml. After molecular sieve chromatography of the sera, one peak of hGH-immunoreactive material of high molecular weight (> 160 kD) and one at the elution volume of monomeric hGH were determined with Pas and Mabs. The major part of the high molecular weight hGH (> 160 kD) seemed to consist of 22-kD hGH molecules, since Pas and all Mabs detected the hGH immunoreactivity (> 160 kD) in a similar manner. This high molecular weight hGH (> 160 kD) was distinguishable from the identified, receptor-like hGH-binding protein in serum.
在对健康受试者血清进行分子筛色谱分析前后，用六种单克隆抗体（Mabs）和一种多克隆抗血清（Pas）对人生长激素（hGH）进行了分析。用Pas测定，他们的hGH水平在<0.2至0.4 ng/ml之间。六种单克隆抗体与五个不同的表位发生反应，并与对应于氨基酸序列15 - 125的hGH片段结合。其中两种单克隆抗体与20-kD hGH的结合减少。单克隆抗体与hGH二聚体形式的结合情况各不相同。用单克隆抗体测定的未分级血清中的人GH水平为<3.1 - 390 ng/ml。血清经分子筛色谱分析后，用Pas和单克隆抗体测定到一个高分子量（>160 kD）的hGH免疫反应性物质峰和一个在单体hGH洗脱体积处的峰。高分子量hGH（>160 kD）的主要部分似乎由22-kD hGH分子组成，因为Pas和所有单克隆抗体以相似的方式检测到hGH免疫反应性（>160 kD）。这种高分子量hGH（>160 kD）与血清中已鉴定的受体样hGH结合蛋白不同。
