Stolar M W, Baumann G
Metabolism. 1986 Jan;35(1):75-7. doi: 10.1016/0026-0495(86)90099-5.
Large molecular weight (oligomeric) forms of human growth hormone (hGH) are present in both pituitary extracts and plasma, but the origin of the plasma forms is not known. We used a monoclonal anti-hGH antibody (No. NA-71), previously employed to characterize hGH oligomers in pituitary extracts, to examine circulating hGH oligomers. This monoclonal antibody discriminated between monomeric and polymeric hGH forms in the pituitary. Plasma from normal subjects and acromegalic patients was fractionated by Sephadex G-100 chromatography and fractions were assayed by a monoclonal radioimmunoassay (RIA) using antibody NA-71 and by a polyclonal RIA. The oligomeric plasma hGH fraction showed decreased immunoreactivity in the monoclonal RIA, similar to that observed with pituitary oligomers. The immunopotency ratios of the various plasma hGH size isomers, as determined in the two RIAs, were virtually identical to those of pituitary hGH size isomers. We conclude that circulating hGH oligomers are immunochemically indistinguishable from pituitary hGH oligomers, and thus are most likely derived from pituitary stores.
人生长激素（hGH）的大分子（寡聚体）形式存在于垂体提取物和血浆中，但血浆中这些形式的来源尚不清楚。我们使用了一种单克隆抗hGH抗体（编号NA-71），该抗体先前用于鉴定垂体提取物中的hGH寡聚体，以检测循环中的hGH寡聚体。这种单克隆抗体能够区分垂体中单体和聚合形式的hGH。通过Sephadex G-100色谱法对正常受试者和肢端肥大症患者的血浆进行分级分离，并使用抗体NA-71通过单克隆放射免疫测定法（RIA）和多克隆RIA对各组分进行检测。寡聚体血浆hGH组分在单克隆RIA中显示出免疫反应性降低，这与垂体寡聚体的情况相似。在两种RIA中测定的各种血浆hGH大小异构体的免疫效价比与垂体hGH大小异构体的几乎相同。我们得出结论，循环中的hGH寡聚体在免疫化学上与垂体hGH寡聚体无法区分，因此很可能来源于垂体储存。
