Castellani P, Viale G, Dorcaratto A, Nicolo G, Kaczmarek J, Querze G, Zardi L
Laboratory of Cell Biology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
Int J Cancer. 1994 Dec 1;59(5):612-8. doi: 10.1002/ijc.2910590507.
Different fibronectin (FN) isoforms are generated by the alternative splicing of 3 regions (ED-A, ED-B and IIICS) of the primary transcript. The FN isoform containing the ED-B sequence, a complete type-III-homology repeat, while having extremely restricted distribution in normal adult tissues, reveals high expression in fetal and tumor tissues. Using the monoclonal antibody (MAb) BC-I, specific for the FN isoform containing the ED-B sequence (B+.FN), we demonstrated here, using immunohistochemical techniques, that while this FN isoform is undetectable in mature vessels, it is highly expressed during angiogenesis both in neoplastic and in normal tissues, as in the case of the functional layer of endometrium during the proliferative phase. B+.FN is thus a marker for the formation of new vessels, and the BC-I MAb may be a useful reagent for evaluating the level of the angiogenetic process in different neoplasms.
不同的纤连蛋白（FN）异构体是由初级转录本的3个区域（ED-A、ED-B和IIICS）的可变剪接产生的。含有ED-B序列的FN异构体是一个完整的III型同源重复序列，在正常成体组织中的分布极为有限，但在胎儿和肿瘤组织中高表达。我们使用对含有ED-B序列的FN异构体（B+.FN）具有特异性的单克隆抗体（MAb）BC-I，通过免疫组织化学技术证明，虽然这种FN异构体在成熟血管中无法检测到，但在肿瘤组织和正常组织的血管生成过程中均高表达，例如增殖期子宫内膜功能层的情况。因此，B+.FN是新血管形成的标志物，而BC-I单克隆抗体可能是评估不同肿瘤血管生成过程水平的有用试剂。
