A simple and inexpensive chromatographic method for the purification of gamma-globulin from human serum.

Inexpensive cation- and anion-exchangers based on continuous polymer beds were prepared directly in a plastic syringe. These beds are unique in the sense that they are synthesized by a very simple and cost-effective procedure; for instance, no preparation of beads is required as in conventional methods. Highly purified gamma-globulin could be prepared easily by step-wise elution accomplished by forcing buffers of increasing salt concentration through the column with the aid of the plunger.