Mukaida N, Okamoto S, Ishikawa Y, Matsushima K
Department of Pharmacology, Kanazawa University, Ishikawa, Japan.
J Leukoc Biol. 1994 Nov;56(5):554-8.
A potent leukocyte chemotactic and activating cytokine, interleukin-8 (IL-8), is produced by numerous types of cells in response to inflammatory stimuli. Accumulating evidence indicate that the transcription of IL-8 gene requires the activation of either the combination of NF-kappa B and AP-1 or that of NF-kappa B and NF-IL6, depending on the type of cells. Alternatively, the activation of NF-kappa B is indispensable for IL-8 gene activation in any types of cells examined. On the other hand, an immunosuppressant, FK506, and a glucocorticoid inhibit the gene transcription as well as the production of IL-8. Molecular analyses of IL-8 gene repression by these agents revealed that both affected the activity of the transcription factor(s) bound to the NF-kappa B site, albeit in different ways, thereby suppressing IL-8 gene transcription. Collectively, IL-8 production seems to be controlled mainly at the activation step of the transcription factor(s) bound to the NF-kappa B site.
白细胞介素-8（IL-8）是一种强效的白细胞趋化和激活细胞因子，由多种类型的细胞在炎症刺激下产生。越来越多的证据表明，IL-8基因的转录需要激活NF-κB和AP-1的组合，或者NF-κB和NF-IL6的组合，这取决于细胞类型。另外，在任何所检测的细胞类型中，NF-κB的激活对于IL-8基因的激活都是必不可少的。另一方面，免疫抑制剂FK506和糖皮质激素会抑制IL-8的基因转录以及产生。对这些药物抑制IL-8基因的分子分析表明，两者都以不同方式影响与NF-κB位点结合的转录因子的活性，从而抑制IL-8基因转录。总体而言，IL-8的产生似乎主要在与NF-κB位点结合的转录因子的激活步骤受到控制。
