Shah R M, Cheng K M, Feeley E J
Department of Oral Biology, University of British Columbia, Vancouver, Canada.
J Exp Zool. 1994 Nov 1;270(3):285-91. doi: 10.1002/jez.1402700307.
A study was undertaken to examine the growth of normal and 5-fluorouracil-treated quail secondary palate during embryogenesis. The rates of DNA, RNA, and protein synthesis were measured in the developing quail palate by liquid scintillation counting of radiolabelled thymidine, uridine, or leucine. In addition, shelf volume was determined morphometrically. The results showed that in control palates the shelf volume increased rapidly between days 5 and 7 of incubation. Drug treatment on day 4 did not alter the shelf volume until day 9 of incubation, at which time the treated shelves were smaller than controls. In control palates, the rate of DNA synthesis decreased steadily between days 5 and 9 of incubation. A burst in RNA synthesis on day 7 of incubation was followed by an increase in protein synthesis. Administration of FU seems to exert its effect via disturbing the synthesis of RNA and protein, instead of disruption of DNA synthesis, to ultimately affect the shelf area, and thus palate morphogenesis in quail. Comparison of avian and mammalian data indicated that differences in their palate morphogenesis are also reflected in the different temporal patterns of various macromolecular synthesis.
进行了一项研究，以检查正常鹌鹑和经5-氟尿嘧啶处理的鹌鹑在胚胎发育过程中次生腭的生长情况。通过对放射性标记的胸腺嘧啶核苷、尿苷或亮氨酸进行液体闪烁计数，来测量发育中的鹌鹑腭中DNA、RNA和蛋白质的合成速率。此外，通过形态测量法确定腭架体积。结果表明，在对照腭中，腭架体积在孵化第5天至第7天之间迅速增加。在第4天进行药物处理，直到孵化第9天才改变腭架体积，此时经处理的腭架比对照腭架小。在对照腭中，DNA合成速率在孵化第5天至第9天之间稳步下降。在孵化第7天RNA合成爆发，随后蛋白质合成增加。给予氟尿嘧啶似乎是通过干扰RNA和蛋白质的合成，而不是破坏DNA合成，最终影响腭架面积，从而影响鹌鹑的腭形态发生。鸟类和哺乳动物数据的比较表明，它们腭形态发生的差异也反映在各种大分子合成的不同时间模式上。
