Young A V, Hehn B M, Cheng K M, Shah R M
Department of Oral Biology, University of British Columbia, Vancouver, Canada.
Histol Histopathol. 1994 Jul;9(3):515-23.
A comparative study was undertaken to investigate the effects of 5-fluorouracil (FU) on glycosaminoglycans (GAG) synthesis during morphogenesis of the secondary palate in birds (where, unlike mammals, palate morphogenesis begins in a horizontal direction ad initium and lacks mammalian-type shelf reorientation) and mammal. Previous studies have shown that FU induces cleft palate in both birds and mammals. Air sacs of quail eggs were injected with 100 micrograms FU in 0.1 ml saline or 0.1 ml saline only. Hamsters were given intramuscular injection of 81 mg/kg FU in 1 ml saline or 1 ml saline only. Total GAG synthesis was measured by incorporation of 3H-glucosamine. Sulfated and non-sulfated GAGs were identified by Alcian Blue histochemistry combined with the use of GAG-degrading enzymes. The results indicated that a continuous synthesis of GAG at a steady rate was associated with normal palate morphogenesis in both quail and hamster. The amount of GAG synthesized in hamster palate was four-fold higher than in quail palate. In contrast to the developing hamster palate where the predominant GAG was hyaluronate, the major GAGs present during quail palate development were sulfated and were concentrated on the nasal side. FU treatment did not affect the rate of GAG synthesis in the developing palate of quail. In contrast, FU administration altered the rates of GAG synthesis, and affected hyaluronate accumulation, during palate morphogenesis in hamster.(ABSTRACT TRUNCATED AT 250 WORDS)
开展了一项比较研究，以调查5-氟尿嘧啶（FU）对鸟类（与哺乳动物不同，鸟类的腭部形态发生最初是水平方向的，且缺乏哺乳动物型的腭架重新定向）和哺乳动物次生腭形态发生过程中糖胺聚糖（GAG）合成的影响。先前的研究表明，FU在鸟类和哺乳动物中均会诱发腭裂。向鹌鹑蛋的气囊中注射0.1 ml盐水中含100微克FU或仅注射0.1 ml盐水。给仓鼠肌肉注射1 ml盐水中含81 mg/kg FU或仅注射1 ml盐水。通过掺入3H-葡糖胺来测量总GAG合成。通过阿尔辛蓝组织化学结合使用GAG降解酶来鉴定硫酸化和非硫酸化的GAG。结果表明，在鹌鹑和仓鼠中，以稳定速率持续合成GAG与正常的腭部形态发生相关。仓鼠腭部合成的GAG量比鹌鹑腭部高四倍。与发育中的仓鼠腭部中主要的GAG是透明质酸不同，鹌鹑腭部发育过程中存在的主要GAG是硫酸化的，且集中在鼻侧。FU处理不影响鹌鹑发育中腭部的GAG合成速率。相比之下，在仓鼠腭部形态发生过程中，施用FU改变了GAG合成速率，并影响了透明质酸的积累。（摘要截短为250字）
