探寻答案。DNA探针在传染病中的应用。
Probing for answers. Use of DNA probes in infectious diseases.
作者信息
Sinnott J T, Ragsdale R, Bergen G A, Padios M M
机构信息
Division of Infectious Diseases and Tropical Medicine, University of South Florida College of Medicine, Tampa.
出版信息
J Fla Med Assoc. 1994 Aug;81(8):543-5.
Diagnostic molecular biology is at the forefront in the diagnosis of infectious diseases. Through the use of deoxyribonucleic acid (DNA) probes in conjunction with other molecular techniques such as polymerase chain reaction (PCR), physicians will be able to more rapidly and accurately diagnose causative agents in a variety of infectious diseases. This article will focus on the molecular basis of these techniques, how they may aid the physician in patient diagnosis, and the shortcomings of this new and exciting technology.
诊断分子生物学在传染病诊断领域处于前沿地位。通过将脱氧核糖核酸（DNA）探针与其他分子技术（如聚合酶链反应（PCR））结合使用，医生将能够更快速、准确地诊断各种传染病的病原体。本文将重点探讨这些技术的分子基础、它们如何帮助医生进行患者诊断，以及这项令人兴奋的新技术的缺点。
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