Rodríguez M, Schudel A A
Institute of Virology, Instituto Nacional de Tecnología Agropecuaria, Moron, Argentina.
Rev Sci Tech. 1993 Jun;12(2):385-423.
The authors describe and summarise the use of nucleic acid hybridisation and polymerase chain reaction (PCR) technologies in the diagnosis of animal diseases. PCR is a powerful biological tool which enables exponential enzymatic amplification in vitro of a given deoxyribonucleic acid sequence. This technique is currently used to study the molecular pathogenesis of many infectious diseases and also for diagnosis. PCR is usually more sensitive than conventional methods and does not require complex facilities, and will therefore soon become the preferred technology of laboratory diagnosticians, field veterinarians and health officers.
作者描述并总结了核酸杂交和聚合酶链反应（PCR）技术在动物疾病诊断中的应用。PCR是一种强大的生物学工具，能够在体外对特定的脱氧核糖核酸序列进行指数级酶促扩增。该技术目前用于研究许多传染病的分子发病机制，也用于诊断。PCR通常比传统方法更灵敏，且不需要复杂的设备，因此很快将成为实验室诊断人员、现场兽医和卫生官员的首选技术。
