Trotter M J, Tron V A
Department of Pathology, Vancouver General Hospital, B.C., Canada.
J Pathol. 1994 Aug;173(4):341-5. doi: 10.1002/path.1711730409.
Lentigo maligna (LM) may represent a tumour arrested in an in situ phase, lacking the angiogenic capacity and underlying dermal neovascularization required for invasive growth. The acquisition of an angiogenic phenotype might be associated with the development of lentigo maligna melanoma (LMM). To investigate this thesis, sections of formalin-fixed, paraffin-embedded tissue from 15 LMMs, and 11 LM excision specimens were stained with the vascular endothelial marker Ulex europaeus agglutinin I. Dermal vessels were counted and vascular morphometry was performed. In specimens in which LMM was present, dermal vascularity was significantly increased in LM compared with normal skin. The most significant increases were found for dermal vascular density (39 per cent increase, P = 0.008) and for total vessel surface area (62 per cent increase, P = 0.005). However, when no LMM was present, the vascular density underlying LM (79 +/- 9 vessels/mm2) did not differ significantly from that of adjacent normal skin (67 +/- 6 vessels/mm2), although focal 'hot spots' of increased vascularity were present. We conclude that increased dermal vascularity is present beneath in situ LM and that this increased vascular density is closely associated with the presence of invasive LMM in the same specimen.
恶性雀斑样痣（LM）可能代表一种处于原位阶段的肿瘤，缺乏侵袭性生长所需的血管生成能力和真皮深层新生血管形成。血管生成表型的获得可能与恶性雀斑样痣黑色素瘤（LMM）的发生有关。为了研究这一论点，对15例LMM和11例LM切除标本的福尔马林固定、石蜡包埋组织切片用血管内皮标记荆豆凝集素I进行染色。对真皮血管进行计数并进行血管形态测定。在存在LMM的标本中，与正常皮肤相比，LM中的真皮血管明显增多。真皮血管密度（增加39%，P = 0.008）和血管总面积（增加62%，P = 0.005）增加最为显著。然而，当不存在LMM时，尽管存在血管增多的局灶性“热点”，但LM下方的血管密度（79±9个血管/mm2）与相邻正常皮肤（67±6个血管/mm2）相比无显著差异。我们得出结论，原位LM下方存在真皮血管增多，且这种增加的血管密度与同一标本中侵袭性LMM的存在密切相关。
