Terpe H J, Koopmann R, Imhof B A, Günthert U
Zentrum für Pathologie, Justus-Liebig-Universität Giessen, Germany.
J Pathol. 1994 Oct;174(2):89-100. doi: 10.1002/path.1711740205.
Low- and high-grade malignant non-Hodgkin's lymphomas have been investigated by immunohistochemistry for their expression of various integrins and CD44 isoforms. Comparison with the expression patterns obtained in non-malignant adult lymph nodes revealed the following differences: alpha 6 and beta 4 integrins were expressed in several high-grade malignant lymphomas to a lower degree than in both the low-grade malignant lymphomas and the normal lymph nodes; all other integrins (alpha 2, alpha 4, alpha 5, alpha v, beta 1, beta 2, beta 3, and beta 7) did not exhibit significant differences in the expression levels between malignant and non-malignant tissues. The standard isoform of CD44 (CD44s) was highly expressed in all lymphoid tissues. Using CD44 exons-specific monoclonal antibodies, CD44 variant isoforms were not detected in non-malignant lymph nodes and were detected only rarely in low-grade malignant lymphomas. In contrast, high-grade malignant lymphomas expressed several CD44 variant isoforms, which included the products from the variant exons 3v, 6v, and 9v, but not 4v. Specifically, detection of exon 3v and 6v products indicates a more aggressive phenotype.
已通过免疫组织化学研究了低级别和高级别恶性非霍奇金淋巴瘤中各种整合素和CD44亚型的表达情况。与非恶性成人淋巴结中的表达模式进行比较，发现了以下差异：α6和β4整合素在几种高级别恶性淋巴瘤中的表达程度低于低级别恶性淋巴瘤和正常淋巴结；所有其他整合素（α2、α4、α5、αv、β1、β2、β3和β7）在恶性和非恶性组织之间的表达水平没有显著差异。CD44的标准亚型（CD44s）在所有淋巴组织中均高表达。使用CD44外显子特异性单克隆抗体，在非恶性淋巴结中未检测到CD44变异亚型，在低级别恶性淋巴瘤中也仅很少检测到。相比之下，高级别恶性淋巴瘤表达几种CD44变异亚型，包括来自变异外显子3v、6v和9v的产物，但不包括4v。具体而言，外显子3v和6v产物的检测表明具有更具侵袭性的表型。
