Amerongen H M, Wilson G A, Fields B N, Neutra M R
GI Cell Biology Laboratory, Children's Hospital, Boston, Massachusetts 02115.
J Virol. 1994 Dec;68(12):8428-32. doi: 10.1128/JVI.68.12.8428-8432.1994.
Reovirus adheres specifically to apical membranes of mouse intestinal M cells and exploits M-cell transepithelial transport activity to enter Peyer's patch mucosa, where replication occurs. Proteolytic conversion of native reovirus to intermediate subviral particles (ISVPs) occurs in the intestine, but it is not known whether conversion is essential for interaction of virus with M cells. We tested the capacity of native virions, ISVPs, and cores (that lack outer capsid proteins) to bind to intestinal epithelial cells in vivo and found that only ISVPs adhered to M cells. Thus, intraluminal conversion of native reovirus to ISVPs is a prerequisite for M-cell adherence, and outer capsid proteins unique to ISVPs (either sigma 1 or products of mu 1) mediate interaction of virus with M-cell apical membranes.
呼肠孤病毒特异性地黏附于小鼠肠道M细胞的顶端膜，并利用M细胞的跨上皮运输活性进入派尔集合淋巴结黏膜，在那里进行复制。天然呼肠孤病毒在肠道中发生蛋白水解转化为中间亚病毒颗粒（ISVP），但尚不清楚这种转化对于病毒与M细胞的相互作用是否至关重要。我们测试了天然病毒粒子、ISVP和核心颗粒（缺乏外 capsid 蛋白）在体内与肠道上皮细胞结合的能力，发现只有ISVP黏附于M细胞。因此，天然呼肠孤病毒在管腔内转化为ISVP是M细胞黏附的先决条件，并且ISVP特有的外 capsid 蛋白（sigma 1或mu 1的产物）介导病毒与M细胞顶端膜的相互作用。
