原发性胆汁性肝硬化患者血清中细菌热休克蛋白60抗体的检测与鉴定
Detection and characterization of antibodies to bacterial heat-shock protein 60 in sera of patients with primary biliary cirrhosis.
作者信息
Yamaguchi H, Miura H, Ohsumi K, Ishimi N, Taguchi H, Ishiyama N, Shiraishi Y, Yamamoto T, Ogata S
机构信息
Department of Microbiology, Kyorin University School of Medicine, Tokyo, Japan.
出版信息
Microbiol Immunol. 1994;38(6):483-7. doi: 10.1111/j.1348-0421.1994.tb01813.x.
The enzyme-linked immunosorbent assay (ELISA) with bacterial heat-shock protein 60 (HSP60) purified from Yersinia enterocolitica (Ye) revealed that the antibodies directed against YeHSP60 existed in sera of patients with primary biliary cirrhosis (PBC). To characterize the epitope specificity of the antibodies in patients, the epitope mapping of HSP60 by means of the antibodies was performed. The results have suggested that the epitope recognized with anti-HSP60 antibodies in PBC relates to the amino acid sequence of YeHSP60 molecule as follows: DLGQAKRVVINKDTTIIIDGVGDEAAIQGRLAQIRQQIEEATSDYDKEK.
用从小肠结肠炎耶尔森菌（Ye）纯化的细菌热休克蛋白60（HSP60）进行的酶联免疫吸附测定（ELISA）显示，原发性胆汁性肝硬化（PBC）患者血清中存在针对小肠结肠炎耶尔森菌热休克蛋白60（YeHSP60）的抗体。为了鉴定患者体内抗体的表位特异性，利用这些抗体对HSP60进行了表位定位。结果表明，PBC中抗HSP60抗体识别的表位与YeHSP60分子的氨基酸序列相关，如下所示：DLGQAKRVVINKDTTIIIDGVGDEAAIQGRLAQIRQQIEEATSDYDKEK。
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