神经母细胞瘤N18TG - 2和胶质瘤C6Bu - 1中[3H]咯利普兰结合与磷酸二酯酶活性
[3H]Rolipram binding and phosphodiesterase activity in neuroblastoma N18TG-2 and glioma C6Bu-1.
作者信息
Tohda M, Murayama T, Hasegawa H, Nogiri S, Nomura Y
机构信息
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
出版信息
Neurosci Lett. 1994 Jul 4;175(1-2):89-91. doi: 10.1016/0304-3940(94)91085-5.
To clarify the functional significance of type IV phosphodiesterase (PDEIV) in neurons and glia cells [3H]rolipram binding and phosphodiesterase (PDE) activity were examined in cytosol and membrane fractions of neuroblastoma N18TG-2 and glioma C6Bu-1 cells. [3H]Rolipram binding was highly observed in cytosolic fractions of N18TG-2 compared to C6Bu-1. Binding was hardly observed in membrane fractions of both types of cells. Rolipram strongly (70%) inhibited the hydrolytic activity of cAMP in cytosolic fractions of N18TG-2. The activity in cytosol fractions of C6Bu-1 was slightly (20%) inhibited by rolipram. These results suggest that PDEIV plays important roles in neurons.
为阐明IV型磷酸二酯酶（PDEIV）在神经元和神经胶质细胞中的功能意义，我们检测了神经母细胞瘤N18TG - 2和胶质瘤C6Bu - 1细胞的胞质溶胶和膜组分中的[3H]咯利普兰结合及磷酸二酯酶（PDE）活性。与C6Bu - 1相比，在N18TG - 2的胞质溶胶组分中高度观察到[3H]咯利普兰结合。在这两种细胞的膜组分中几乎未观察到结合。咯利普兰强烈（70%）抑制N18TG - 2胞质溶胶组分中cAMP的水解活性。咯利普兰对C6Bu - 1胞质溶胶组分中的活性有轻微（20%）抑制作用。这些结果表明PDEIV在神经元中起重要作用。
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