Christensen N D, Kirnbauer R, Schiller J T, Ghim S J, Schlegel R, Jenson A B, Kreider J W
Department of Pathology, Milton S. Hershey Medical Center, Hershey, Pennsylvania 17033.
Virology. 1994 Nov 15;205(1):329-35. doi: 10.1006/viro.1994.1649.
Antibodies reactive to HPV types 6 and 11 were tested in ELISA and HPV-11 neutralization assays to determine whether these closely related types shared cross-reactive neutralizing epitopes. A series of HPV-11 neutralizing monoclonal antibodies (N-MAbs) that targeted conformational epitopes on infectious HPV-11 and HPV-11 L1 virus-like particles (VLPs) were tested for type-specificity of reactivity using intact HPV-6 L1 VLPs. Polyclonal antisera generated against intact HPV-6 L1 VLPs were also tested for HPV-11 neutralizing capacity using the athymic mouse xenograft system. The results demonstrated that conformationally dependent neutralizing epitopes on HPV-11 were very type-specific. Three of the four HPV-11 N-MAbs were negative for binding to HPV-6 L1 VLP, and the fourth demonstrated binding to HPV-6 L1 VLPs that was several orders of magnitude weaker than its binding to HPV-11 L1 VLP. The polyclonal anti-HPV-6 L1 VLP antiserum was only partially protective against HPV-11 infectivity even at a low dilution of 1:100. In contrast, polyclonal anti-HPV-11 L1 VLP antiserum was completely protective at dilutions greater than 1:10,000.
在ELISA和HPV-11中和试验中检测了对HPV 6型和11型有反应的抗体，以确定这些密切相关的类型是否共享交叉反应性中和表位。使用完整的HPV-6 L1病毒样颗粒（VLP），对一系列靶向感染性HPV-11和HPV-11 L1病毒样颗粒上构象表位的HPV-11中和单克隆抗体（N-MAb）进行反应性的型特异性检测。还使用无胸腺小鼠异种移植系统检测了针对完整HPV-6 L1病毒样颗粒产生的多克隆抗血清的HPV-11中和能力。结果表明，HPV-11上依赖构象的中和表位具有很强的型特异性。四种HPV-11 N-MAb中的三种与HPV-6 L1 VLP结合呈阴性，第四种与HPV-6 L1 VLP 的结合比其与HPV-11 L1 VLP的结合弱几个数量级。即使在1:100的低稀释度下，多克隆抗HPV-6 L1 VLP抗血清对HPV-11感染性也只有部分保护作用。相比之下，多克隆抗HPV-11 L1 VLP抗血清在大于1:10,000的稀释度下具有完全保护作用。
