Christensen N D, Reed C A, Cladel N M, Hall K, Leiserowitz G S
Department of Pathology, Milton S. Hershey Medical Center, Hershey, Pennsylvania 17033, USA.
Virology. 1996 Oct 15;224(2):477-86. doi: 10.1006/viro.1996.0554.
A set of 13 monoclonal antibodies (MAbs) was generated against HPV-6 L1 virus-like particles (VLPs), screened for reactivity to HPV-6 and HPV-11 L1 VLPs by ELISA, and tested for neutralization of HPV-11 infection. Both cross-reactive and type-specific epitopes were detected such that 4 of 13 MAbs reacted to surface conformational sites on HPV-6 L1 VLPs and the remaining 9 MAbs were cross-reactive to both HPV-6 and HPV-11 L1 VLPs. four of the 9 cross-reactive MAbs were able to neutralize HPV-11 infectivity, and 3 of 4 of these neutralizing MAbs (N-MAbs) identified shared surface conformational sites. One N-MAb therefore recognized a surface linear epitope as judged by positive binding to L1 in a Western blot assay. The neutralization status of these cross-reactive MAbs with regard to HPV-6 could not be assayed. These results demonstrated that the closely related HPV types 6 and 11 contain both type-specific and shared neutralizing epitopes.
针对人乳头瘤病毒6型（HPV-6）L1病毒样颗粒（VLP）产生了一组13种单克隆抗体（MAb），通过酶联免疫吸附测定（ELISA）筛选其与人乳头瘤病毒6型和11型（HPV-11）L1 VLP的反应性，并测试其对HPV-11感染的中和作用。检测到了交叉反应性表位和型特异性表位，13种MAb中有4种与人乳头瘤病毒6型L1 VLP的表面构象位点发生反应，其余9种MAb与人乳头瘤病毒6型和11型L1 VLP均发生交叉反应。9种交叉反应性MAb中有4种能够中和HPV-11的感染性，其中4种中和性MAb（N-MAb）中有3种识别出共同的表面构象位点。因此，通过蛋白质印迹分析中与L1的阳性结合判断，一种N-MAb识别出一个表面线性表位。无法测定这些交叉反应性MAb对HPV-6的中和状态。这些结果表明，密切相关的HPV-6型和11型既包含型特异性中和表位，也包含共同的中和表位。
