Dikranian K, Trosheva M, Nikolov S, Bodin P
Department of Anatomy and Histology, Medical University, Varna, Bulgaria.
Acta Histochem. 1994 Jun;96(2):145-53. doi: 10.1016/S0065-1281(11)80170-4.
Localization of nitric oxide synthase (NOS) in endothelial cells of umbilical cord vessels and in cultured human umbilical vein endothelial cells was investigated by light and electron-microscopical (immunogold) immunohistochemistry. We observed localization of NOS-immunoreactivity in the majority (97%) of the endothelial cells of the umbilical vein and in a subpopulation (6.7%) of endothelial cells of the umbilical arteries. NOS was observed as well in the amniotic epithelium and in the cells of Wharton's jelly. Immunogold labelling in human umbilical vein endothelial cells dominated in the cellular matrix and was not associated with cellular organelles. Since human umbilical vessels are unique in lacking innervation, the functional significance of endothelium derived relaxing factor EDRF/NO in the local control of vascular flow is discussed.
通过光学显微镜和电子显微镜（免疫金法）免疫组织化学研究了一氧化氮合酶（NOS）在脐带血管内皮细胞和培养的人脐静脉内皮细胞中的定位。我们观察到，NOS免疫反应性定位于脐静脉的大多数（97%）内皮细胞以及脐动脉的一部分（6.7%）内皮细胞中。在羊膜上皮和华通胶细胞中也观察到了NOS。人脐静脉内皮细胞中的免疫金标记主要位于细胞基质中，与细胞器无关。由于人脐血管缺乏神经支配这一独特性，因此讨论了内皮源性舒张因子EDRF/NO在局部控制血管血流中的功能意义。
