Danckwardt-Lillieström N, Laurent C, Hellström S, Friberg U, Kinnefors A, Rask-Andersen H
Department of Otorhinolaryngology, University Hospital, Uppsala, Sweden.
Acta Otolaryngol. 1994 Jul;114(4):382-6. doi: 10.3109/00016489409126074.
This study was undertaken with the aim of localizing hyaluronan (hyaluronic acid, HYA) in tissue sections of the human endolymphatic sac by use of a hyaluronan-binding affinity protein and the avidin-biotin/peroxidase staining procedure. Five human endolymphatic sacs were removed during surgery for acoustic neuroma. After microwave-aided fixation and decalcification, paraffin-embedded sections were prepared by routine histological methods. HYA was detected in some of the intraluminal substance as well as in parts of the epithelial lining, mainly in the rugose portions of the endolymphatic sac. HYA was observed intracellularly in epithelial cells. It was also found in the subepithelial tissue near the epithelia and close to the bony aqueduct. The distribution of HYA was uneven at all locations. The finding of HYA within the human endolymphatic sac may imply that this substance has important functions in the control of inner ear fluid homeostasis.
本研究旨在通过使用透明质酸结合亲和蛋白和抗生物素蛋白-生物素/过氧化物酶染色程序，在人内淋巴囊组织切片中定位透明质酸（玻尿酸，HYA）。在听神经瘤手术过程中切除了5个人的内淋巴囊。经过微波辅助固定和脱钙后，通过常规组织学方法制备石蜡包埋切片。在一些腔内物质以及部分上皮衬里中检测到了HYA，主要在内淋巴囊的皱折部分。在上皮细胞内观察到了HYA。在靠近上皮且靠近骨导水管的上皮下组织中也发现了HYA。在所有位置，HYA的分布都不均匀。在人内淋巴囊中发现HYA可能意味着该物质在控制内耳液体稳态方面具有重要功能。
