Yan Z Q, Kramer K, Bast A, Timmerman H
Faculty of Chemistry, Department of Pharmacochemistry, Vrije Universeit, Amsterdam, The Netherlands.
Agents Actions. 1994 Jun;41 Spec No:C111-2. doi: 10.1007/BF02007790.
The influence of histamine on nitric oxide synthase (NOS) in the development of airway smooth muscle hyperresponsiveness to histamine was investigated in vitro. In the absence of histamine, NG-nitro-L-arginine methyl ester (L-NAME, 100 microM) had no significant effect on the basal tone. However, precontraction of the tissues with histamine (0.3 microM) resulted in a significant contractile response to L-NAME in the preparations with intact epithelium. Removal of the epithelial layer decreased the responses to L-NAME. L-arginine could partially reverse the contraction produced by L-NAME. L-NAME enhanced the maximal response to histamine, but the sensitivity of the tracheal smooth muscle to histamine was not affected. These results suggest that, in the airway, histamine can activate NOS, resulting in the release of nitric oxide. The latter may be regarded as a local negative modulator to maintain the tissue in a physiological homeostasis.
在体外研究了组胺对气道平滑肌对组胺高反应性发展过程中一氧化氮合酶（NOS）的影响。在无组胺的情况下，NG-硝基-L-精氨酸甲酯（L-NAME，100微摩尔）对基础张力无显著影响。然而，用组胺（0.3微摩尔）预收缩组织会导致完整上皮制备物中对L-NAME产生显著的收缩反应。去除上皮层会降低对L-NAME的反应。L-精氨酸可部分逆转L-NAME产生的收缩。L-NAME增强了对组胺的最大反应，但气管平滑肌对组胺的敏感性未受影响。这些结果表明，在气道中，组胺可激活NOS，导致一氧化氮释放。后者可被视为一种局部负调节剂，以维持组织处于生理稳态。
