Kristensson K, Aldskogius M, Peng Z C, Olsson T, Aldskogius H, Bentivoglio M
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
J Neurocytol. 1994 Aug;23(8):453-9. doi: 10.1007/BF01184069.
Induction of an interferon-gamma-like molecule, previously isolated from neurons (N-IFN-gamma), and of the neuronal isoform I of the synthetic enzyme of the free radical nitric oxide, nitric oxide synthase I, as well as of NADPH-diaphorase, were examined in axotomized dorsal motor vagal and hypoglossal neurons. Unilateral transection of the vagal and hypoglossal nerves was performed in the same rat and an induction of N-IFN-gamma and nitric oxide synthase I immunostaining as well as NADPH-diaphorase histochemical positivity was observed in the ipsilateral motoneurons after 2-4 days. The immuno- and enzyme-histochemical positivities were much stronger in the dorsal motor vagal neurons than in hypoglossal neurons. Two and 4 weeks after axotomy N-IFN-gamma immunoreactivity and NADPH-diaphorase positivity persisted in the former, but started to decrease in the latter neurons. Previous data have shown that 23 weeks after nerve transection the majority of the dorsal motor vagal neurons are lost, while the majority of the hypoglossal neurons survive. The high and persistent expression of N-IFN-gamma and nitric oxide synthase I after axotomy in the dorsal motor vagal neurons, that are largely destined to die, indicates that the co-induction of these two molecules may be implicated in the pathogenesis of neuronal degeneration.
在切断轴突的迷走神经背核运动神经元和舌下神经运动神经元中，检测了一种先前从神经元中分离出的干扰素-γ样分子（N-IFN-γ）、自由基一氧化氮合成酶的神经元同工型I（一氧化氮合酶I）以及NADPH-黄递酶的诱导情况。在同一只大鼠中对迷走神经和舌下神经进行单侧横断，2-4天后在同侧运动神经元中观察到N-IFN-γ和一氧化氮合酶I免疫染色以及NADPH-黄递酶组织化学阳性的诱导。迷走神经背核运动神经元中的免疫和酶组织化学阳性比舌下神经运动神经元中的要强得多。轴突切断后2周和4周，N-IFN-γ免疫反应性和NADPH-黄递酶阳性在前一种神经元中持续存在，但在后一种神经元中开始下降。先前的数据表明，神经横断23周后，大多数迷走神经背核运动神经元死亡，而大多数舌下神经运动神经元存活。在大多注定要死亡的迷走神经背核运动神经元中，轴突切断后N-IFN-γ和一氧化氮合酶I的高表达和持续表达表明，这两种分子的共同诱导可能与神经元变性的发病机制有关。
