对13株1型和3型呼肠孤病毒野毒株S4基因的序列比较分析
Comparative sequence analysis of the reovirus S4 genes from 13 serotype 1 and serotype 3 field isolates.
作者信息
Kedl R, Schmechel S, Schiff L
机构信息
Department of Microbiology, University of Minnesota, Minneapolis 55455.
出版信息
J Virol. 1995 Jan;69(1):552-9. doi: 10.1128/JVI.69.1.552-559.1995.
Abstract
The reovirus sigma 3 protein is a major outer capsid protein that may function to regulate translation within infected cells. To facilitate the understanding of sigma 3 structure and functions and the evolution of mammalian reoviruses, we sequenced cDNA copies of the S4 genes from 10 serotype 3 and 3 serotype 1 reovirus field isolates and compared these sequences with sequences of prototypic strains of the three reovirus serotypes. We found that the sigma 3 proteins are highly conserved: the two longest conserved regions contain motifs proposed to function in binding zinc and double-stranded RNA. We used the 16 viral isolates to investigate the hypothesis that structural interactions between sigma 3 and the cell attachment protein, sigma 1, constrain their evolution and to identify a determinant within sigma 3 that is in close proximity to the sigma 1 hemagglutination site.
摘要
呼肠孤病毒σ3蛋白是一种主要的外衣壳蛋白，可能在受感染细胞内发挥调节翻译的作用。为了便于理解σ3的结构和功能以及哺乳动物呼肠孤病毒的进化，我们对10株3型和3株1型呼肠孤病毒野外分离株的S4基因的cDNA拷贝进行了测序，并将这些序列与三种呼肠孤病毒血清型原型株的序列进行了比较。我们发现σ3蛋白高度保守：两个最长的保守区域包含被认为在结合锌和双链RNA中起作用的基序。我们使用这16株病毒分离株来研究σ3与细胞附着蛋白σ1之间的结构相互作用限制它们进化的假说，并确定σ3中与σ1血凝素位点紧密相邻的一个决定因素。
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本文引用的文献
1
Observations on a newly recognized virus (Abney) of the reovirus family.对呼肠孤病毒科一种新发现病毒（阿布尼病毒）的观察
Am J Hyg. 1960 Mar;71:258-65. doi: 10.1093/oxfordjournals.aje.a120109.
2
Further observations on the natural infection of cattle with reoviruses.关于呼肠孤病毒对牛自然感染的进一步观察
Am J Hyg. 1963 Jan;77:38-48. doi: 10.1093/oxfordjournals.aje.a120294.
3
Analysis of the stimulation of reporter gene expression by the sigma 3 protein of reovirus in co-transfected cells.呼肠孤病毒σ3蛋白在共转染细胞中对报告基因表达的刺激作用分析。
J Gen Virol. 1993 Jun;74 ( Pt 6):1055-62. doi: 10.1099/0022-1317-74-6-1055.
4
Early steps in reovirus infection are associated with dramatic changes in supramolecular structure and protein conformation: analysis of virions and subviral particles by cryoelectron microscopy and image reconstruction.呼肠孤病毒感染的早期步骤与超分子结构和蛋白质构象的显著变化相关：通过冷冻电子显微镜和图像重建对病毒粒子和亚病毒颗粒进行分析。
J Cell Biol. 1993 Sep;122(5):1023-41. doi: 10.1083/jcb.122.5.1023.
5
Protective anti-reovirus monoclonal antibodies and their effects on viral pathogenesis.保护性抗呼肠孤病毒单克隆抗体及其对病毒致病机制的影响。
J Virol. 1993 Jun;67(6):3446-53. doi: 10.1128/JVI.67.6.3446-3453.1993.
6
Sequence diversity within the reovirus S2 gene: reovirus genes reassort in nature, and their termini are predicted to form a panhandle motif.呼肠孤病毒S2基因内的序列多样性：呼肠孤病毒基因在自然界中发生重配，并且预测其末端会形成一个柄环基序。
J Virol. 1994 Feb;68(2):750-6. doi: 10.1128/JVI.68.2.750-756.1994.
7
Mutations in a CCHC zinc-binding motif of the reovirus sigma 3 protein decrease its intracellular stability.呼肠孤病毒σ3蛋白的CCHC锌结合基序中的突变会降低其细胞内稳定性。
J Virol. 1994 Aug;68(8):5287-90. doi: 10.1128/JVI.68.8.5287-5290.1994.
8
The plus strand of reovirus gene S2 is identical with its in vitro transcript.呼肠孤病毒基因S2的正链与其体外转录物相同。
Virology. 1980 Aug;105(1):282-6. doi: 10.1016/0042-6822(80)90181-6.
9
Genetic variation during persistent reovirus infection: presence of extragenically suppressed temperature-sensitive lesions in wild-type virus isolated from persistently infected L cells.呼肠孤病毒持续感染期间的基因变异：从持续感染的L细胞中分离出的野生型病毒中存在基因外抑制的温度敏感损伤。
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10
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