Ikeda H, Nakayama H, Oda T, Kuwano K, Yamaga A, Ueno T, Yoh M, Hiyamuta K, Koga Y, Toshima H
Third Department of Internal Medicine, Kurume University School of Medicine, Japan.
Am Heart J. 1994 Dec;128(6 Pt 1):1091-8. doi: 10.1016/0002-8703(94)90738-2.
We investigated whether percutaneous transluminal coronary angioplasty (PTCA) would induce neutrophil activation in patients with coronary artery disease. Blood samples were taken from the coronary sinus in 14 patients who underwent PTCA and in 9 control subjects who underwent coronary arteriography (CAG). Flow cytometry was used to measure membrane surface expression of beta 2 integrin (CD11b) and the generation of hydrogen peroxide in neutrophils after ex vivo phorbol myristate acetate stimulation by 2,'7'-dichlorofluorescein. Neutrophil elastase was measured by an immunoenzymatic method. Surface expression of CD11b increased significantly, approximately twofold, after PTCA but not after CAG. Mean fluorescence intensity of 2',7'-dichlorofluorescein in stimulated neutrophils decreased significantly after PTCA, suggesting a previous in vivo activation, but not after CAG. Neutrophil elastase increased significantly after PTCA but not after CAG. These data indicate that PTCA induces neutrophil activation and suggest that neutrophils may contribute to the ischemic injury.
我们研究了经皮腔内冠状动脉成形术（PTCA）是否会诱导冠心病患者的中性粒细胞活化。从14例行PTCA的患者及9例行冠状动脉造影（CAG）的对照受试者的冠状窦采集血样。采用流式细胞术检测β2整合素（CD11b）的膜表面表达以及在体外经佛波酯肉豆蔻酸酯乙酸酯刺激后中性粒细胞中过氧化氢的生成，通过2,7'-二氯荧光素进行检测。采用免疫酶法测定中性粒细胞弹性蛋白酶。PTCA后CD11b的表面表达显著增加，约为两倍，但CAG后未增加。PTCA后，刺激的中性粒细胞中2,7'-二氯荧光素的平均荧光强度显著降低，提示先前存在体内活化，但CAG后未出现此情况。PTCA后中性粒细胞弹性蛋白酶显著增加，但CAG后未增加。这些数据表明PTCA可诱导中性粒细胞活化，并提示中性粒细胞可能导致缺血性损伤。
