Baj Z, Kowalski J, Kantorski J, Pokoca L, Kośmider M, Pawlicki L, Tchórzewski H
Department of Pathophysiology and Immunology, Military Medical Academy, Lódź, Poland.
Atherosclerosis. 1994 Apr;106(2):159-68. doi: 10.1016/0021-9150(94)90121-x.
We examined the influence of transient myocardial ischemia on the number and function of neutrophils in patients with effort angina (EA). We tested fluorometrically the expression of neutrophil membrane molecules (CD11b, CD11c, CD18) and neutrophil oxidative burst using a chemiluminescence (CL) generation system. The estimations were conducted before, 1 min after and 20 min after percutaneous transluminal coronary angioplasty (PTCA) in 15 patients qualified for the treatment because of single-vessel disease. Eight EA patients subjected to coronary arteriography (CA) comprised a control group. We did not observe any marked changes in leucocytosis or lymphocyte number in peripheral blood (PB) or in coronary sinus blood (CSB) after the procedure. The percentage of granulocytes in coronary blood decreased significantly 20 min after reperfusion. No significant changes in white blood cell count were noted in peripheral blood of PTCA patients or in control CA subjects. Oxidative burst of nonstimulated and fMLP, PMA and zymosan stimulated sinus blood neutrophils was significantly depressed 1 min after inflation, and enhanced 20 min after reperfusion. We found a significant increase in the percentage of the CD11c+ neutrophils from 56.7 +/- 7.4% to 64 +/- 6.5% 20 min after inflation and postischemic decrease in the CD11c molecule expression on CSB neutrophils. Significant positive linear correlation (Rval = 0.71) between inflation time and the CD11c molecule expression on CSB immediately after reperfusion was also noted. The results may reflect local activation of neutrophils in ischemic myocardium as a response to ischemia induced increase of activating stimuli.
我们研究了短暂性心肌缺血对劳力性心绞痛（EA）患者中性粒细胞数量及功能的影响。我们使用化学发光（CL）生成系统，通过荧光法检测中性粒细胞膜分子（CD11b、CD11c、CD18）的表达及中性粒细胞的氧化爆发。对因单支血管病变符合治疗条件的15例患者，在经皮腔内冠状动脉成形术（PTCA）前、术后1分钟及术后20分钟进行评估。8例接受冠状动脉造影（CA）的EA患者组成对照组。术后，我们未观察到外周血（PB）或冠状窦血（CSB）中白细胞增多或淋巴细胞数量有任何明显变化。再灌注20分钟后，冠状血中粒细胞百分比显著下降。PTCA患者外周血及对照组CA受试者的白细胞计数均未发现显著变化。球囊扩张1分钟后，未刺激及经fMLP、PMA和酵母聚糖刺激的窦血中性粒细胞的氧化爆发显著受抑，而再灌注20分钟后增强。我们发现球囊扩张20分钟后，CD11c + 中性粒细胞百分比从56.7 +/- 7.4%显著增加至64 +/- 6.5%，且缺血后CSB中性粒细胞上CD11c分子表达下降。还注意到球囊扩张时间与再灌注后即刻CSB上CD11c分子表达之间存在显著正线性相关（R值 = 0.71）。这些结果可能反映了缺血心肌中中性粒细胞的局部激活，作为对缺血诱导的激活刺激增加的一种反应。
