对照及阿尔茨海默病（AD）成纤维细胞中2-5A合成酶基因的mRNA水平和甲基化模式
mRNA levels and methylation patterns of the 2-5A synthetase gene in control and Alzheimer's disease (AD) fibroblasts.
作者信息
An S, Khanna K K, Wu J M
机构信息
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
出版信息
Biochem Mol Biol Int. 1994 Aug;33(5):835-40.
We have examined the mRNA levels and methylation patterns of the interferon-inducible 2',5'-oligoadenylate (2-5A) synthetase gene in skin fibroblasts derived from AD and age/sex-matched control subjects. Northern or slot hybridization analysis of total RNA showed a 63% and 46% decrease in the steady state level of 2-5A synthetase mRNA in AD cells as compared to controls, following a 24 h and 48 h treatment with IFN-beta ser. The 2-5A synthetase gene as studied by Southern analysis using the methylation-sensitive restriction enzymes HpaII and Msp I was found to be hypomethylated in AD cells. No difference in methylation patterns of the actin gene existed between control and AD fibroblasts.
我们检测了来自特应性皮炎（AD）患者以及年龄/性别匹配的对照受试者的皮肤成纤维细胞中干扰素诱导的2',5'-寡腺苷酸（2-5A）合成酶基因的mRNA水平和甲基化模式。对总RNA进行的Northern或狭缝杂交分析显示，在用IFN-β血清处理24小时和48小时后，与对照相比，AD细胞中2-5A合成酶mRNA的稳态水平分别降低了63%和46%。使用对甲基化敏感的限制性内切酶HpaII和Msp I通过Southern分析研究发现，2-5A合成酶基因在AD细胞中发生了低甲基化。对照和成纤维细胞的肌动蛋白基因甲基化模式没有差异。
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